TY - JOUR
T1 - Effects of high-fat diet on plasma profiles of eicosanoid metabolites in mice
AU - Wang, Weicang
AU - Yang, Jun
AU - Yang, Haixia
AU - Sanidad, Katherine Z.
AU - Hammock, Bruce D.
AU - Kim, Daeyoung
AU - Zhang, Guodong
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Obesity is a serious health problem in the US and is associated with increased risks of various human diseases. To date, the mechanisms by which obesity increases the risks of a wide range of human diseases are not well understood. Here we used a LC–MS/MS-based lipidomics, which can analyze >100 bioactive lipid mediators produced by cyclooxygenase, lipoxygenase, and cytochrome P450 enzymes, to analyze plasma profiles of lipid mediators in high-fat diet induced obesity in C57BL/6 mice. Our results show that the plasma concentrations of epoxyoctadecenoic acids (EpOMEs, also termed as leukotoxins) are significantly increased in plasma of high-fat diet-fed mice, in addition, EpOMEs are among the most abundant lipid mediators detected in mouse plasma. Since substantial studies have shown that EpOMEs and their metabolites have a large array of detrimental effects on health, enhanced levels of EpOMEs could contribute to the pathology of obesity.
AB - Obesity is a serious health problem in the US and is associated with increased risks of various human diseases. To date, the mechanisms by which obesity increases the risks of a wide range of human diseases are not well understood. Here we used a LC–MS/MS-based lipidomics, which can analyze >100 bioactive lipid mediators produced by cyclooxygenase, lipoxygenase, and cytochrome P450 enzymes, to analyze plasma profiles of lipid mediators in high-fat diet induced obesity in C57BL/6 mice. Our results show that the plasma concentrations of epoxyoctadecenoic acids (EpOMEs, also termed as leukotoxins) are significantly increased in plasma of high-fat diet-fed mice, in addition, EpOMEs are among the most abundant lipid mediators detected in mouse plasma. Since substantial studies have shown that EpOMEs and their metabolites have a large array of detrimental effects on health, enhanced levels of EpOMEs could contribute to the pathology of obesity.
KW - Eicosanoids
KW - Epoxyoctadecenoic acids
KW - High-fat diet
KW - Leukotoxins
KW - Obesity
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U2 - 10.1016/j.prostaglandins.2016.11.003
DO - 10.1016/j.prostaglandins.2016.11.003
M3 - Article
C2 - 27913146
AN - SCOPUS:85003875955
VL - 127
SP - 9
EP - 13
JO - Journal of Lipid Mediators and Cell Signalling
JF - Journal of Lipid Mediators and Cell Signalling
SN - 1098-8823
ER -