Effects of hepatic inducers on testicular epoxide-metabolizing enzymes in the rat and mouse

Karen W. Dibiasio, Marilyn H. Silva, Bruce D. Hammock, Lee R. Shull

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3 Scopus citations

Abstract

Effects of Hepatic Inducers on Testicular Epoxide-Metabolizing Enzymes in the Rat and Mouse. DIBIASIO, K. W., SILVA, M. H., HAMMOCK B. D., AND SHULL, L. R. (1989). Fundam. Appl. Toxicol. 12, 449-459. Testicular toxicants have become of increasing importance, necessitating a better understanding of the possible role of testicular xenobiotic metabolism. The responsiveness of testicular microsomal epoxide hydrolase (mEH), cytosolic epoxide hydrolase (cEH), and cytosolic glutathione S-transferase (cGST to hepatic inducers was studied in sexually mature male F344 rats and CD-1 mice. The hepatic inducers employed were phenobarbital (PB), β-naphthoflavone (BNF), and butylated hydcoxyanisole (BHA) which are known to induce cytochrome P-450, cytochrome P-448, and cGST, respectively. Hepatic mEH, cEH, and cGST activities were assessed as positive controls. Measurable activities of all enzymes studied were present in the testes of both rats and mice. PB, BNF, and BHA produced the expected effects on mEH, cEH, and cGST in rat and mouse livers, whereas the testes were generally nonresponsive to the inducers. Induction of testicular cGST by PB occurred in mice but not rats and was the only testicular effect produced by the hepatic inducers in this study.

Original languageEnglish (US)
Pages (from-to)449-459
Number of pages11
JournalToxicological Sciences
Volume12
Issue number3
DOIs
StatePublished - Apr 1989

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ASJC Scopus subject areas

  • Molecular Biology
  • Embryology
  • Cell Biology
  • Genetics
  • Developmental Biology
  • Toxicology

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