Effects of hepatic inducers on testicular epoxide-metabolizing enzymes in the rat and mouse

Karen W. DiBiasio, Marilyn H. Silva, Bruce D. Hammock, Lee R. Shull

Research output: Contribution to journalArticle

Abstract

Testicular toxicants have become of increasing importance, necessitating a better understanding of the possible role of testicular xenobiotic metabolism. The responsiveness of testicular microsomal epoxide hydrolase (mEH) cytosolic epoxide hydrolase (cEH), and cytosolic glutathione S-transferase (cGST) to hepatic inducers was studied in sexually mature male F344 rats and CD-1 mice. The hepatic inducers employed were phenobarbital (PB), β-naphthoflavone (BNF), and butylated hydroxyanisole (BHA) which are known to induce cytochrome P-450, cytochrome P-448, and cGST, respectively. Hepatic mEH, cEH, and cGST activities were assessed as positive controls. Measurable activities of all enzymes studied were present in the testes of both rats and mice. PB, BNF, and BHA produced the expected effects on mEH, cEH, and cGST in rat and mouse livers, whereas the testes were generally nonresponsive to the inducers. Induction of testicular cGST by PB occurred in mice but not rats and was the only testicular effect produced by the hepatic inducers in this study.

Original languageEnglish (US)
Pages (from-to)449-459
Number of pages11
JournalFundamental and Applied Toxicology
Volume12
Issue number3
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Toxicology

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