Effects of growth factors and L-arginine on ischemic skin flaps in rats.

C. M. Walls, C. R. Gregory, L. S. Beck, J. P. Cooke, Stephen M Griffey, Philip H Kass

Research output: Contribution to journalArticle

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Abstract

This study determined the effect of the polypeptide growth factors transforming growth factor-beta (TGF-beta), insulin-like growth factor-I (IGF-I), and growth hormone (GH) alone and in combination with dietary L-Arginine HCL (ARG) on skin flap survival in rats. Caudally based dorsal skin flaps were created in 110 Sprague-Dawley rats. The rats were randomly assigned into three treatment groups, based on drinking water supplementation. Group 1 (n = 50) received ARG in their drinking water, group 2 (n = 50) received tap water alone, and group 3 (n = 10) received N-omega-nitro-L-arginine (L-NA) and hydralazine. Groups 1 and 2 were divided into subgroups of 10 rats each based on treatment with either: TGF-beta, IGF-I, GH, or IGF-I + GH. All subgroups that received GH had significantly greater (P < .0001) median body weight gains when compared with subgroups not receiving GH. L-arginine HCL when added to IGF-I negated the positive effects of IGF-I on both flap survival and weight gain. Although the rats in all subgroups from groups 1 and 2 had an increase in mean percent skin flap survival when compared with the water alone subgroup, only rats receiving IGF-I, or the combination of ARG with either TGF-beta or GH, had statistically significant enhanced skin flap survival. Rats in group 3 did not show an increase in skin flap survival when compared with the control subgroup.

Original languageEnglish (US)
Pages (from-to)484-491
Number of pages8
JournalVeterinary surgery : VS : the official journal of the American College of Veterinary Surgeons
Volume24
Issue number6
StatePublished - Nov 1995

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Insulin-Like Growth Factor I
growth factors
Growth Hormone
arginine
insulin-like growth factor I
Arginine
Intercellular Signaling Peptides and Proteins
somatotropin
Skin
rats
Transforming Growth Factor beta
transforming growth factor beta
Drinking Water
Weight Gain
drinking water
Hydralazine
weight gain
Water
Nitroarginine
tap water

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Effects of growth factors and L-arginine on ischemic skin flaps in rats. / Walls, C. M.; Gregory, C. R.; Beck, L. S.; Cooke, J. P.; Griffey, Stephen M; Kass, Philip H.

In: Veterinary surgery : VS : the official journal of the American College of Veterinary Surgeons, Vol. 24, No. 6, 11.1995, p. 484-491.

Research output: Contribution to journalArticle

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