Effects of FGF-1 and FGF-2 on GD3 immunoreactive spinal neuroepithelial cells

P. G. Bannerman, T. M. Oliver, Z. Xu, A. Shieh, David E Pleasure

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Embryonic central nervous system neuroepithelial cells are a transient population of cells that give rise to neuronal and glial progenitors. In the E12-E16 embryonic rat spinal neural tube we have identified neuroepithelial cells as radially oriented cells expressing the GD3 ganglioside as recognized by the monoclonal anti-GD3 ganglioside antibodies, R24 and LB1. In vitro, neuroepithelial cells, which migrate from the ventral aspect of El2 rat lumbosacral neural tube explants, also express GD3 ganglioside immunoreactivity, thus permitting their distinction from neural crest cells (NCC) which migrate from the dorsal aspect of such explants. Fibroblast growth factor-1 (FGF-1, acidic FGF) and FGF-2 (basic FGF) increase the migration of neuroepithelial cells and the extent to which they incorporate the thymidine analogue bromodeoxyuridine (BrdU). They do not, however, alter the rate at which these migrating neuroepithelial cells undergo cell death. Previous observations established the actions of FGF-1 and FGF-2 on neuronal and glial cells. The present study indicates that these growth factors also influence the motility and proliferation of progenitor cells at a developmental stage which precedes their divergence into neuronal and glial lineages.

Original languageEnglish (US)
Pages (from-to)549-557
Number of pages9
JournalJournal of Neuroscience Research
Volume45
Issue number5
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Neuroepithelial Cells
Fibroblast Growth Factor 1
Fibroblast Growth Factor 2
Neuroglia
Neural Tube
Neural Crest
Bromodeoxyuridine
Thymidine
Intercellular Signaling Peptides and Proteins
Cell Death
Stem Cells
Central Nervous System
Antibodies
Population
GD3 ganglioside

Keywords

  • fibroblast growth factors
  • GD3 ganglioside
  • mitogen
  • neuroepithelial cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effects of FGF-1 and FGF-2 on GD3 immunoreactive spinal neuroepithelial cells. / Bannerman, P. G.; Oliver, T. M.; Xu, Z.; Shieh, A.; Pleasure, David E.

In: Journal of Neuroscience Research, Vol. 45, No. 5, 1996, p. 549-557.

Research output: Contribution to journalArticle

Bannerman, P. G. ; Oliver, T. M. ; Xu, Z. ; Shieh, A. ; Pleasure, David E. / Effects of FGF-1 and FGF-2 on GD3 immunoreactive spinal neuroepithelial cells. In: Journal of Neuroscience Research. 1996 ; Vol. 45, No. 5. pp. 549-557.
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