Effects of facultative symbionts and heat stress on the metabolome of pea aphids

Gaelen Burke, Oliver Fiehn, Nancy Moran

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

We examined metabolite pools of pea aphids with different facultative symbiont infections, and characterized their effects on aphid metabolism in baseline and heat stress conditions. The bacterial symbiont Serratia symbiotica protects aphid hosts from the detrimental results of heat stress and shields the obligate symbiont Buchnera from effects of heat. We investigated whether broad effects on metabolism might correlate with this protection. Both facultative symbiont infection and heat treatment had large effects on the aphid metabolome. All three pea aphid facultative symbionts had similar effects on aphid metabolism despite their evolutionary diversity. Paradoxically, heat triggers lysis of many S. symbiotica cells and a correlated rapid reduction in S. symbiotica titres within aphid hosts. We conclude that facultative symbionts can have substantial effects on host metabolic pools, and we hypothesize that the protective effects of S. symbiotica may reflect the delivery of protective metabolites to aphid or Buchnera cells, after heat exposure.

Original languageEnglish (US)
Pages (from-to)242-252
Number of pages11
JournalISME Journal
Volume4
Issue number2
DOIs
StatePublished - Feb 2010

Fingerprint

metabolome
Aphids
Acyrthosiphon pisum
Metabolome
Peas
symbiont
aphid
symbionts
heat stress
Aphidoidea
Hot Temperature
Buchnera (Proteobacteria)
Buchnera
heat
metabolism
metabolites
Serratia
metabolite
infection
protective effect

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

Effects of facultative symbionts and heat stress on the metabolome of pea aphids. / Burke, Gaelen; Fiehn, Oliver; Moran, Nancy.

In: ISME Journal, Vol. 4, No. 2, 02.2010, p. 242-252.

Research output: Contribution to journalArticle

Burke, Gaelen ; Fiehn, Oliver ; Moran, Nancy. / Effects of facultative symbionts and heat stress on the metabolome of pea aphids. In: ISME Journal. 2010 ; Vol. 4, No. 2. pp. 242-252.
@article{df16f34a07cf4ff4be71cae6e4e0821c,
title = "Effects of facultative symbionts and heat stress on the metabolome of pea aphids",
abstract = "We examined metabolite pools of pea aphids with different facultative symbiont infections, and characterized their effects on aphid metabolism in baseline and heat stress conditions. The bacterial symbiont Serratia symbiotica protects aphid hosts from the detrimental results of heat stress and shields the obligate symbiont Buchnera from effects of heat. We investigated whether broad effects on metabolism might correlate with this protection. Both facultative symbiont infection and heat treatment had large effects on the aphid metabolome. All three pea aphid facultative symbionts had similar effects on aphid metabolism despite their evolutionary diversity. Paradoxically, heat triggers lysis of many S. symbiotica cells and a correlated rapid reduction in S. symbiotica titres within aphid hosts. We conclude that facultative symbionts can have substantial effects on host metabolic pools, and we hypothesize that the protective effects of S. symbiotica may reflect the delivery of protective metabolites to aphid or Buchnera cells, after heat exposure.",
author = "Gaelen Burke and Oliver Fiehn and Nancy Moran",
year = "2010",
month = "2",
doi = "10.1038/ismej.2009.114",
language = "English (US)",
volume = "4",
pages = "242--252",
journal = "ISME Journal",
issn = "1751-7362",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Effects of facultative symbionts and heat stress on the metabolome of pea aphids

AU - Burke, Gaelen

AU - Fiehn, Oliver

AU - Moran, Nancy

PY - 2010/2

Y1 - 2010/2

N2 - We examined metabolite pools of pea aphids with different facultative symbiont infections, and characterized their effects on aphid metabolism in baseline and heat stress conditions. The bacterial symbiont Serratia symbiotica protects aphid hosts from the detrimental results of heat stress and shields the obligate symbiont Buchnera from effects of heat. We investigated whether broad effects on metabolism might correlate with this protection. Both facultative symbiont infection and heat treatment had large effects on the aphid metabolome. All three pea aphid facultative symbionts had similar effects on aphid metabolism despite their evolutionary diversity. Paradoxically, heat triggers lysis of many S. symbiotica cells and a correlated rapid reduction in S. symbiotica titres within aphid hosts. We conclude that facultative symbionts can have substantial effects on host metabolic pools, and we hypothesize that the protective effects of S. symbiotica may reflect the delivery of protective metabolites to aphid or Buchnera cells, after heat exposure.

AB - We examined metabolite pools of pea aphids with different facultative symbiont infections, and characterized their effects on aphid metabolism in baseline and heat stress conditions. The bacterial symbiont Serratia symbiotica protects aphid hosts from the detrimental results of heat stress and shields the obligate symbiont Buchnera from effects of heat. We investigated whether broad effects on metabolism might correlate with this protection. Both facultative symbiont infection and heat treatment had large effects on the aphid metabolome. All three pea aphid facultative symbionts had similar effects on aphid metabolism despite their evolutionary diversity. Paradoxically, heat triggers lysis of many S. symbiotica cells and a correlated rapid reduction in S. symbiotica titres within aphid hosts. We conclude that facultative symbionts can have substantial effects on host metabolic pools, and we hypothesize that the protective effects of S. symbiotica may reflect the delivery of protective metabolites to aphid or Buchnera cells, after heat exposure.

UR - http://www.scopus.com/inward/record.url?scp=76349093143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76349093143&partnerID=8YFLogxK

U2 - 10.1038/ismej.2009.114

DO - 10.1038/ismej.2009.114

M3 - Article

C2 - 19907504

AN - SCOPUS:76349093143

VL - 4

SP - 242

EP - 252

JO - ISME Journal

JF - ISME Journal

SN - 1751-7362

IS - 2

ER -