Effects of exposure to environmental tobacco smoke on a human tracheobronchial epithelial cell line

Weimin Sun, Reen Wu, Jerold A Last

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

BEAS-2B cells, a human bronchial epithelial line immortalized by viral transformation, were exposed to sidestream tobacco smoke (STS) as a surrogate for environmental tobacco smoke (ETS) under biphasic culture conditions where the apical portion of the cells was in direct contact with the gas phase. Dose-dependent cytotoxicity was observed. In addition, induction of an as yet uncharacterized protein of molecular weight 45 000 was associated with exposure to STS. This protein might be part of a protective response of exposed cells, which do not show a classical heat shock response when exposed to STS. We conclude that STS and ETS can be directly cytotoxic to human airway epithelial cells in biphasic culture at concentrations not unreasonable for smoky indoor atmospheres. The model system described in this paper should be useful for studying the detailed mechanisms of cytotoxicity of, and protection from, ETS exposure in the human cells most directly exposed to ETS in vivo.

Original languageEnglish (US)
Pages (from-to)163-174
Number of pages12
JournalToxicology
Volume100
Issue number1-3
DOIs
StatePublished - Jun 26 1995

Keywords

  • Cell toxicity
  • Lung disease
  • Passive smoking
  • Protein synthesis
  • Tobacco smoke

ASJC Scopus subject areas

  • Toxicology

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