Effects of exopolysaccharide production on liquid vegetative growth, stress survival, and stationary phase recovery in Myxococcus xanthus

Wei Hu, Jing Wang, Ian Howard Mchardy, Renate Lux, Zhe Yang, Yuezhong Li, Wenyuan Shi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Exopolysaccharide (EPS) of Myxococcus xanthus is a well-regulated cell surface component. In addition to its known functions for social motility and fruiting body formation on solid surfaces, EPS has also been proposed to play a role in multi-cellular clumping in liquid medium, though this phenomenon has not been well studied. In this report, we confirmed that M. xanthus clumps formed in liquid were correlated with EPS levels and demonstrated that the EPS encased cell clumps exhibited biofilm-like structures. The clumps protected the cells at physiologically relevant EPS concentrations, while cells lacking EPS exhibited significant reduction in long-term viability and resistance to stressful conditions. However, excess EPS production was counterproductive to vegetative growth and viable cell recovery declined in extended late stationary phase as cells became trapped in the matrix of clumps. Therefore, optimal EPS production by M. xanthus is important for normal physiological functions in liquid.

Original languageEnglish (US)
Pages (from-to)241-248
Number of pages8
JournalJournal of Microbiology
Volume50
Issue number2
DOIs
StatePublished - Apr 2012

Fingerprint

Myxococcus xanthus
Growth
Cellular Structures
Biofilms

Keywords

  • exopolysaccharide
  • Myxococcus xanthus
  • stationary phase recovery
  • stress survival
  • vegetative growth

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Effects of exopolysaccharide production on liquid vegetative growth, stress survival, and stationary phase recovery in Myxococcus xanthus. / Hu, Wei; Wang, Jing; Mchardy, Ian Howard; Lux, Renate; Yang, Zhe; Li, Yuezhong; Shi, Wenyuan.

In: Journal of Microbiology, Vol. 50, No. 2, 04.2012, p. 241-248.

Research output: Contribution to journalArticle

Hu, Wei ; Wang, Jing ; Mchardy, Ian Howard ; Lux, Renate ; Yang, Zhe ; Li, Yuezhong ; Shi, Wenyuan. / Effects of exopolysaccharide production on liquid vegetative growth, stress survival, and stationary phase recovery in Myxococcus xanthus. In: Journal of Microbiology. 2012 ; Vol. 50, No. 2. pp. 241-248.
@article{19fa053381844dea849399499027db6b,
title = "Effects of exopolysaccharide production on liquid vegetative growth, stress survival, and stationary phase recovery in Myxococcus xanthus",
abstract = "Exopolysaccharide (EPS) of Myxococcus xanthus is a well-regulated cell surface component. In addition to its known functions for social motility and fruiting body formation on solid surfaces, EPS has also been proposed to play a role in multi-cellular clumping in liquid medium, though this phenomenon has not been well studied. In this report, we confirmed that M. xanthus clumps formed in liquid were correlated with EPS levels and demonstrated that the EPS encased cell clumps exhibited biofilm-like structures. The clumps protected the cells at physiologically relevant EPS concentrations, while cells lacking EPS exhibited significant reduction in long-term viability and resistance to stressful conditions. However, excess EPS production was counterproductive to vegetative growth and viable cell recovery declined in extended late stationary phase as cells became trapped in the matrix of clumps. Therefore, optimal EPS production by M. xanthus is important for normal physiological functions in liquid.",
keywords = "exopolysaccharide, Myxococcus xanthus, stationary phase recovery, stress survival, vegetative growth",
author = "Wei Hu and Jing Wang and Mchardy, {Ian Howard} and Renate Lux and Zhe Yang and Yuezhong Li and Wenyuan Shi",
year = "2012",
month = "4",
doi = "10.1007/s12275-012-1349-5",
language = "English (US)",
volume = "50",
pages = "241--248",
journal = "Journal of Microbiology",
issn = "1225-8873",
publisher = "Microbiological Society of Korea",
number = "2",

}

TY - JOUR

T1 - Effects of exopolysaccharide production on liquid vegetative growth, stress survival, and stationary phase recovery in Myxococcus xanthus

AU - Hu, Wei

AU - Wang, Jing

AU - Mchardy, Ian Howard

AU - Lux, Renate

AU - Yang, Zhe

AU - Li, Yuezhong

AU - Shi, Wenyuan

PY - 2012/4

Y1 - 2012/4

N2 - Exopolysaccharide (EPS) of Myxococcus xanthus is a well-regulated cell surface component. In addition to its known functions for social motility and fruiting body formation on solid surfaces, EPS has also been proposed to play a role in multi-cellular clumping in liquid medium, though this phenomenon has not been well studied. In this report, we confirmed that M. xanthus clumps formed in liquid were correlated with EPS levels and demonstrated that the EPS encased cell clumps exhibited biofilm-like structures. The clumps protected the cells at physiologically relevant EPS concentrations, while cells lacking EPS exhibited significant reduction in long-term viability and resistance to stressful conditions. However, excess EPS production was counterproductive to vegetative growth and viable cell recovery declined in extended late stationary phase as cells became trapped in the matrix of clumps. Therefore, optimal EPS production by M. xanthus is important for normal physiological functions in liquid.

AB - Exopolysaccharide (EPS) of Myxococcus xanthus is a well-regulated cell surface component. In addition to its known functions for social motility and fruiting body formation on solid surfaces, EPS has also been proposed to play a role in multi-cellular clumping in liquid medium, though this phenomenon has not been well studied. In this report, we confirmed that M. xanthus clumps formed in liquid were correlated with EPS levels and demonstrated that the EPS encased cell clumps exhibited biofilm-like structures. The clumps protected the cells at physiologically relevant EPS concentrations, while cells lacking EPS exhibited significant reduction in long-term viability and resistance to stressful conditions. However, excess EPS production was counterproductive to vegetative growth and viable cell recovery declined in extended late stationary phase as cells became trapped in the matrix of clumps. Therefore, optimal EPS production by M. xanthus is important for normal physiological functions in liquid.

KW - exopolysaccharide

KW - Myxococcus xanthus

KW - stationary phase recovery

KW - stress survival

KW - vegetative growth

UR - http://www.scopus.com/inward/record.url?scp=84860370023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860370023&partnerID=8YFLogxK

U2 - 10.1007/s12275-012-1349-5

DO - 10.1007/s12275-012-1349-5

M3 - Article

C2 - 22538652

AN - SCOPUS:84860370023

VL - 50

SP - 241

EP - 248

JO - Journal of Microbiology

JF - Journal of Microbiology

SN - 1225-8873

IS - 2

ER -