Effects of estrogen and aging on the synaptic distribution of phosphorylated Akt-immunoreactivity in the CA1 region of the female rat hippocampus

Murat Yildirim, William G.M. Janssen, W. Y.Wendy Lou, Keith T. Akama, Bruce S. McEwen, Teresa A. Milner, John Morrison

Research output: Contribution to journalArticle

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Abstract

The estrogen 17β-estradiol (E) increases the axospinous synaptic density and plasticity in the hippocampal CA1 region of young female rats but fails to do so in aged female rats. This E stimulus on synaptic plasticity is associated with the phosphorylation-dependent activation of Akt kinase. Our previous findings demonstrated that increased estrogen levels subsequently increase phosphorylated Akt (pAkt)-immunoreactivity (-IR) within the dendritic shafts and spines of pyramidal neurons in young female rats. Therefore, because Akt can promote cell survival and growth, we tested the hypothesis that the less plastic synapses of aged female rats would contain less E-stimulated pAkt-IR. Here, young (3-4 months) and aged (22-23 months) female rats were ovariectomized 7 days prior to a 48-h administration of either vehicle or E. The pAkt-IR synaptic distribution was then analyzed using post-embedding electron microscopy. In both young and aged rats, pAkt-IR was found in dendritic spines and terminals, and pAkt-IR was particularly abundant at the post-synaptic density. Quantitative analyses revealed that the percentage of pAkt-labeled synapses was significantly greater in young rats compared to aged rats. Nonetheless, E treatment significantly increased pAkt-IR in pre- and post-synaptic profiles of both young and aged rats, although the stimulus in young rats was notably more widespread. These data support the evidence that hormone-activated signaling associated with cell growth and survival is diminished in the aged brain. However, the observation that E can still increase pAkt-IR in aged synapses presents this signaling component as a candidate target for hormone replacement therapies.

Original languageEnglish (US)
Pages (from-to)98-108
Number of pages11
JournalBrain Research
Volume1379
DOIs
StatePublished - Mar 16 2011
Externally publishedYes

Fingerprint

Hippocampus
Estrogens
Synapses
Dendritic Spines
Neuronal Plasticity
Cell Survival
Post-Synaptic Density
Hippocampal CA1 Region
Pyramidal Cells
Hormone Replacement Therapy
Growth
Plastics
Estradiol
Electron Microscopy
Phosphotransferases
Phosphorylation
Hormones
Brain

Keywords

  • Akt
  • Dendritic spine
  • Electron microscopy
  • Reproductive senescence
  • Signaling molecules
  • Synapses

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Effects of estrogen and aging on the synaptic distribution of phosphorylated Akt-immunoreactivity in the CA1 region of the female rat hippocampus. / Yildirim, Murat; Janssen, William G.M.; Lou, W. Y.Wendy; Akama, Keith T.; McEwen, Bruce S.; Milner, Teresa A.; Morrison, John.

In: Brain Research, Vol. 1379, 16.03.2011, p. 98-108.

Research output: Contribution to journalArticle

Yildirim, Murat ; Janssen, William G.M. ; Lou, W. Y.Wendy ; Akama, Keith T. ; McEwen, Bruce S. ; Milner, Teresa A. ; Morrison, John. / Effects of estrogen and aging on the synaptic distribution of phosphorylated Akt-immunoreactivity in the CA1 region of the female rat hippocampus. In: Brain Research. 2011 ; Vol. 1379. pp. 98-108.
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