Effects of epigallocatechin gallate on regulatory T cell number and function in obese v. lean volunteers

Jung Mi Yun, Ishwarlal Jialal, Sridevi Devaraj

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Obesity predisposes to an increased incidence of diabetes and CVD. Also, obesity is a pro-inflammatory state. Regulatory T cells (Tregs) are essential negative regulators of inflammation and are down-regulated in pro-inflammatory states. Animal models of obesity are associated with decreased Tregs. The dietary modulation of Tregs could be used as a therapeutic strategy to control inflammation. Epigallocatechin gallate (EGCG) is a potent anti-inflammatory agent and an active ingredient of green tea and is suggested to have a role as a preventive agent in obesity, diabetes and CVD. The role of EGCG in the modulation of Tregs has, however, not been studied. Thus, the aim of the present study was to determine the effect of EGCG on the number and function of Tregs in obese and lean human subjects in vitro, and to delineate its specific regulation mechanisms. Tregs were isolated from normal-weight and obese subjects. Tregs were cultured in the absence or presence of EGCG (20m) for 24h. Foxp3-expressing Tregs were enumerated using flow cytometry. Histone deacetylase (HDAC) activity and nuclear NF-Bp65 level were measured by ELISA and Western blots. Obese subjects had lower Tregs and IL-10 production than lean subjects. EGCG treatment significantly enhanced the number of Foxp3-expressing Tregs and IL-10 production in vitro (P<0.05) in both groups. Also, EGCG decreased NF-B activity and increased HDAC activity and HDAC-2 expression in Tregs (P<0.05) in both groups. Thus, in part, EGCG enhances the functionality of Tregs, i.e. IL-10 production and number by suppressing the NF-B signalling pathway via inducing epigenetic changes.

Original languageEnglish (US)
Pages (from-to)1771-1777
Number of pages7
JournalBritish Journal of Nutrition
Volume103
Issue number12
DOIs
StatePublished - Jun 2010

Fingerprint

Regulatory T-Lymphocytes
Volunteers
Cell Count
Obesity
Interleukin-10
Histone Deacetylases
Histone Deacetylase 2
Inflammation
Tea
epigallocatechin gallate
Epigenomics
Flow Cytometry
Anti-Inflammatory Agents
Animal Models
Western Blotting
Enzyme-Linked Immunosorbent Assay
Weights and Measures
Incidence
Therapeutics

Keywords

  • Epigenetics
  • Green tea
  • Inflammation
  • Regulatory T cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

Effects of epigallocatechin gallate on regulatory T cell number and function in obese v. lean volunteers. / Yun, Jung Mi; Jialal, Ishwarlal; Devaraj, Sridevi.

In: British Journal of Nutrition, Vol. 103, No. 12, 06.2010, p. 1771-1777.

Research output: Contribution to journalArticle

Yun, Jung Mi ; Jialal, Ishwarlal ; Devaraj, Sridevi. / Effects of epigallocatechin gallate on regulatory T cell number and function in obese v. lean volunteers. In: British Journal of Nutrition. 2010 ; Vol. 103, No. 12. pp. 1771-1777.
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