Effects of early postnatal alcohol exposure on the developing retinogeniculate projections in C57BL/6 mice

Ilknur Dursun, Ewa Jakubowska-Doĝru, Birsen Elibol-Can, Deborah van der List, Barbara Chapman, Lihong Qi, Robert F Berman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Previous studies on the adverse effects of perinatal exposure to ethanol (EtOH) on the developing visual system mainly focused on retinal and optic nerve morphology. The aim of the present study was to investigate whether earlier reported retinal and optic nerve changes are accompanied by anomalies in eye-specific fiber segregation in the dorsal lateral geniculate nucleus (dLGN). C57BL/6 mice pups were exposed to ethanol by intragastric intubation at either 3 or 4 g/kg from postnatal days (PD) 3-10, the third trimester equivalent to human gestation. Control (C) and intubation control (IC) groups not exposed to ethanol were included. On PD9, retinogeniculate projections were labeled by intraocular microinjections of cholera toxin-β (CTB) either conjugated to Alexa 488 (green) or 594 (red) administrated to the left and right eye, respectively. Pups were sacrificed 24 h after the last CTB injection. The results showed that ethanol exposure decreased the total number of dLGN neurons and significantly reduced the total dLGN projection as well as the contralateral and ipsilateral projection areas.

Original languageEnglish (US)
Pages (from-to)173-179
Number of pages7
JournalAlcohol
Volume47
Issue number3
DOIs
StatePublished - May 2013

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Keywords

  • Anterograde labeling of retinogeniculate projections
  • Eye-specific segregation
  • Mice
  • Postnatal ethanol
  • Stereological cell counts
  • The dorsal lateral geniculate nucleus (dLGN)

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neurology
  • Toxicology
  • Health(social science)

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