Effects of early house dust mite exposure on the immunologic response in nasal mucosal cells of rhesus macaque monkeys

Jonathan Liang, Justin Fontaine, Rebecca Fishman, Joan Gerriets, Lisa Miller

Research output: Contribution to journalArticlepeer-review


OBJECTIVE: The airway remodeling concept proposes that respiratory mucosa responds to allergens and toxins though inflammatory and immune-mediated responses that may persist even after exposure removal. House dust must (HDM) is a common allergen and a major cause of allergic disease. We aim to understand the effects of early house dust mite exposure in altering the immunologic response of nasal mucosal cells. STUDY DESIGN: Infant rhesus macaque monkeys were exposed to filtered air (FA) or episodic HDM. Animals were exposed to 11 3-day cycles of HDM during the first 6 months of life. All animals were raised in filtered air conditions until 3 years of age. METHODS: Sinonasal cells were harvested with necropsy. Cell cultures were grown, and cells were exposed to HDM in vitro at 10 ug/ml, 50 ug/ml, and 100 ug/ml. Enzymelinked immunosorbent assay (ELISA) was performed on cell apical and basolateral supernatant to measure IL-6 and IL-8 protein expression. RESULTS: HDM animals (n = 3) and FA animals (n = 5) showed dosedependent differences in relative IL-6 expression both apically and basolaterally. HDM animals showed significant increased relative IL-6 apical expression (p = 0.021) and decreased relative IL-6 basolateral expression (p = 0.033) at a high concentration of HDM exposure. No significant differences were seen in relative IL-8 expression between the treatment groups. CONCLUSION: Early HDM exposure increases relative apical IL-6 expression with high levels of HDM exposure. This effect is not seen with IL-8 expression. Early life allergen exposure may lead to apical remodeling in nasal mucosal cells with a persistent or enhanced IL-6 mediated immune response.

Original languageEnglish (US)
Issue numberSUPPL. 3
StatePublished - Oct 2010

ASJC Scopus subject areas

  • Otorhinolaryngology


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