Effects of dietary decosahexaenoic acid (DHA) on eNOS in human coronary artery endothelial cells

Charles L Stebbins, James P. Stice, C. Michael Hart, Fiona N. Mbai, Anne A Knowlton

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Endothelial dysfunction occurs in heart disease and may reduce functional capacity via attenuations in peripheral blood flow. Dietary decosahexaenoic acid (DHA) may improve this dysfunction, but the mechanism is unknown. This study determined if DHA enhances expression and activity of eNOS in cultured human coronary artery endothelial cells (HCAEC). HCAEC from 4 donors were treated with 5 nM, 50 nM, or 1 μM DHA for 7 days to model chronic DHA exposure. A trend for increased expression of endothelial nitric oxide synthase (eNOS) and phospho-eNOS was observed with 5 and 50 nM DHA. DHA also enhanced expression of 2 proteins instrumental in activation of eNOS: phospho-Akt (5 and 50 nM) and HSP90 (50 nM and 1 μM). Vascular endothelial growth factor-induced activation of Akt increased NOx in treated (50 nM DHA) versus untreated HCAEC (9.2 ± 1.0 vs 3.3 ± 1.1 μmol/μg protein/μL). Findings suggest that DHA enhances eNOS and Akt activity, augments HSP90 expression, and increases NO bioavailability in response to Akt kinase activation.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalJournal of Cardiovascular Pharmacology and Therapeutics
Volume13
Issue number4
DOIs
StatePublished - Dec 2008

Keywords

  • 3-nitrotyrosine
  • Akt kinase
  • Cell culture
  • HSP90
  • Phospho-Akt
  • Phospho-eNOS

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Cardiology and Cardiovascular Medicine

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