Abstract
Objective - To determine effects of lipoic acid, vitamin E, and cysteine before and after oxidant challenge in cats. Animals - 24 sexually intact adult cats. Procedure - Cats were allocated into 4 equal groups. For 25 weeks, group A was fed a control dry diet and groups B, C, and D received this diet supplemented with vitamin E (2,200 U/kg [dry matter basis {DMB}]) plus cysteine (9.5 g/kg [DMB]), lipoate (150 mg/kg [DMB]), or all 3 antioxidants together, respectively. Weights were measured every 3 days and venous blood obtained every 5 weeks for CBC; serum biochemical analyses; lymphocyte blastogenesis; thiobarbituric acid reactive substances concentration; and concentrations of plasma protein carbonyl, 8-OH d-guanosine, blood glutathione, plasma amino acid, lipoate, and dihydrolipoate. At 15 weeks, all cats received acetaminophen (9 mg/kg, PO, once) clinical effects were observed, and methemoglobin concentrations were measured. Results - Lymphocyte blastogenesis increased transiently in group C and D cats. After acetaminophen administration, all groups had transient increases in methemoglobin within 4 hours and mild, brief facial edema; group C had decreased glutathione concentration and increased 8-CH d-guanosine concentration versus controls; and protein carbonyl concentration increased least for group B. Plasma lipoate and dihydrolipoate concentrations peaked by week 10 for groups C and D. Conclusions and Clinical Relevance - Lipoate, vitamin E, and cysteine did not have synergistic effects. Lipoate supplementation (150 mg/kg [DMB]) did not act as an antioxidant but appeared to enhance oxidant effects of acetaminophen. Vitamin E plus cysteine had protective effects.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 196-204 |
| Number of pages | 9 |
| Journal | American Journal of Veterinary Research |
| Volume | 66 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2005 |
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- veterinary(all)
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Effects of dietary antioxidant supplementation before and after oral acetaminophen challenge in cats. / Hill, Ana S.; Rogers, Quinton R.; O'Neill, Sharron L.; Christopher, Mary M.
In: American Journal of Veterinary Research, Vol. 66, No. 2, 02.2005, p. 196-204.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of dietary antioxidant supplementation before and after oral acetaminophen challenge in cats
AU - Hill, Ana S.
AU - Rogers, Quinton R.
AU - O'Neill, Sharron L.
AU - Christopher, Mary M.
PY - 2005/2
Y1 - 2005/2
N2 - Objective - To determine effects of lipoic acid, vitamin E, and cysteine before and after oxidant challenge in cats. Animals - 24 sexually intact adult cats. Procedure - Cats were allocated into 4 equal groups. For 25 weeks, group A was fed a control dry diet and groups B, C, and D received this diet supplemented with vitamin E (2,200 U/kg [dry matter basis {DMB}]) plus cysteine (9.5 g/kg [DMB]), lipoate (150 mg/kg [DMB]), or all 3 antioxidants together, respectively. Weights were measured every 3 days and venous blood obtained every 5 weeks for CBC; serum biochemical analyses; lymphocyte blastogenesis; thiobarbituric acid reactive substances concentration; and concentrations of plasma protein carbonyl, 8-OH d-guanosine, blood glutathione, plasma amino acid, lipoate, and dihydrolipoate. At 15 weeks, all cats received acetaminophen (9 mg/kg, PO, once) clinical effects were observed, and methemoglobin concentrations were measured. Results - Lymphocyte blastogenesis increased transiently in group C and D cats. After acetaminophen administration, all groups had transient increases in methemoglobin within 4 hours and mild, brief facial edema; group C had decreased glutathione concentration and increased 8-CH d-guanosine concentration versus controls; and protein carbonyl concentration increased least for group B. Plasma lipoate and dihydrolipoate concentrations peaked by week 10 for groups C and D. Conclusions and Clinical Relevance - Lipoate, vitamin E, and cysteine did not have synergistic effects. Lipoate supplementation (150 mg/kg [DMB]) did not act as an antioxidant but appeared to enhance oxidant effects of acetaminophen. Vitamin E plus cysteine had protective effects.
AB - Objective - To determine effects of lipoic acid, vitamin E, and cysteine before and after oxidant challenge in cats. Animals - 24 sexually intact adult cats. Procedure - Cats were allocated into 4 equal groups. For 25 weeks, group A was fed a control dry diet and groups B, C, and D received this diet supplemented with vitamin E (2,200 U/kg [dry matter basis {DMB}]) plus cysteine (9.5 g/kg [DMB]), lipoate (150 mg/kg [DMB]), or all 3 antioxidants together, respectively. Weights were measured every 3 days and venous blood obtained every 5 weeks for CBC; serum biochemical analyses; lymphocyte blastogenesis; thiobarbituric acid reactive substances concentration; and concentrations of plasma protein carbonyl, 8-OH d-guanosine, blood glutathione, plasma amino acid, lipoate, and dihydrolipoate. At 15 weeks, all cats received acetaminophen (9 mg/kg, PO, once) clinical effects were observed, and methemoglobin concentrations were measured. Results - Lymphocyte blastogenesis increased transiently in group C and D cats. After acetaminophen administration, all groups had transient increases in methemoglobin within 4 hours and mild, brief facial edema; group C had decreased glutathione concentration and increased 8-CH d-guanosine concentration versus controls; and protein carbonyl concentration increased least for group B. Plasma lipoate and dihydrolipoate concentrations peaked by week 10 for groups C and D. Conclusions and Clinical Relevance - Lipoate, vitamin E, and cysteine did not have synergistic effects. Lipoate supplementation (150 mg/kg [DMB]) did not act as an antioxidant but appeared to enhance oxidant effects of acetaminophen. Vitamin E plus cysteine had protective effects.
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UR - http://www.scopus.com/inward/citedby.url?scp=14844300132&partnerID=8YFLogxK
U2 - 10.2460/ajvr.2005.66.196
DO - 10.2460/ajvr.2005.66.196
M3 - Article
VL - 66
SP - 196
EP - 204
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 2
ER -