Effects of diet-age and streptomycin on virulence of Autographa californica M nucleopolyhedrovirus against the tobacco budworm

Kelli Hoover, Christine M. Schultz, Stephen S. Lane, Bryony C. Bonning, Bruce D. Hammock, Sean S. Duffey

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Addition of the antibiotic streptomycin to two artificial diets routinely used in bioassays of neonate larvae of Heliothis virescens (tobacco budworm) infected with Autographa californica M nucleopolyhedrovirus (AcMNPV) increased lethal times of the virus. After storage of diets for 3 weeks at 4°C, lethal times of infected larvae were significantly slower compared to those for larvae bioassayed using diets stored for 2 weeks or less. The effect of diet-age on rate of mortality was not the result of a change in total protein content or pH of the diet, but was apparently the result of some other alteration in the quality of the diet (e.g. microbial spoilage, palatibility, and/or nutritional value unrelated to total protein). Although we did not determine why lethal times were slower in response to streptomycin concentration or diet-age, we did find that slower lethal times were correlated with slower relative growth rates (RGR) of infected larvae. In addition, RGR of infected larvae decreased as a function of increasing streptomycin concentration, diet-age, and the interaction of the two factors. These results demonstrate that it is difficult to obtain consistent and comparable bioassay results if antibiotic composition and diet-age are not controlled. We suggest a standardized diet or highly standardized procedures for a given diet be developed that permits comparison of bioassays among and within laboratories.

Original languageEnglish (US)
Pages (from-to)46-50
Number of pages5
JournalJournal of Invertebrate Pathology
Volume69
Issue number1
StatePublished - Jan 1997

Fingerprint

Autographa californica multiple nucleopolyhedrovirus
Heliothis virescens
streptomycin
virulence
tobacco
diet
larva
larvae
bioassay
bioassays
antibiotics
effect
nutritional adequacy
artificial diet
protein
artificial diets
neonate
spoilage
neonates
nutritive value

Keywords

  • Antibiotics
  • Autographa californica nuclear polyhedrosis virus
  • Heliothis virescens
  • Relative growth rate
  • Streptomycin diet-age
  • Virulence

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

Hoover, K., Schultz, C. M., Lane, S. S., Bonning, B. C., Hammock, B. D., & Duffey, S. S. (1997). Effects of diet-age and streptomycin on virulence of Autographa californica M nucleopolyhedrovirus against the tobacco budworm. Journal of Invertebrate Pathology, 69(1), 46-50.

Effects of diet-age and streptomycin on virulence of Autographa californica M nucleopolyhedrovirus against the tobacco budworm. / Hoover, Kelli; Schultz, Christine M.; Lane, Stephen S.; Bonning, Bryony C.; Hammock, Bruce D.; Duffey, Sean S.

In: Journal of Invertebrate Pathology, Vol. 69, No. 1, 01.1997, p. 46-50.

Research output: Contribution to journalArticle

Hoover, K, Schultz, CM, Lane, SS, Bonning, BC, Hammock, BD & Duffey, SS 1997, 'Effects of diet-age and streptomycin on virulence of Autographa californica M nucleopolyhedrovirus against the tobacco budworm', Journal of Invertebrate Pathology, vol. 69, no. 1, pp. 46-50.
Hoover, Kelli ; Schultz, Christine M. ; Lane, Stephen S. ; Bonning, Bryony C. ; Hammock, Bruce D. ; Duffey, Sean S. / Effects of diet-age and streptomycin on virulence of Autographa californica M nucleopolyhedrovirus against the tobacco budworm. In: Journal of Invertebrate Pathology. 1997 ; Vol. 69, No. 1. pp. 46-50.
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