Effects of confinement on the mechanical properties of self-assembled articular cartilage constructs in the direction orthogonal to the confinement surface

Benjamin D. Elder, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

This study examined the effects of radial confinement and passive axial compression-induced vertical confinement, on the biomechanical, biochemical, and histological properties of self-assembled chondrocyte constructs. The self-assembled constructs, engineered without the use of an exogenous scaffold, exhibited significant increases in stiffness in the direction orthogonal to that of the confinement surface. With radial confinement, the significantly increased aggregate modulus was accompanied by increased collagen organization in the direction perpendicular to the articular surface, with no change in collagen or glycosaminoglycan (GAG) content. Additionally, radial confinement was most beneficial when applied before 2 weeks. With passive axial compression, the significantly increased Young's modulus and ultimate tensile strength were accompanied by a significant increase in collagen production. This study is the first to demonstrate the beneficial effects of confinement on tissue engineered constructs in the direction orthogonal to that of the confinement surface.

Original languageEnglish (US)
Pages (from-to)238-246
Number of pages9
JournalJournal of Orthopaedic Research
Volume26
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Keywords

  • Articular cartilage
  • Extracellular matrix
  • Mechanical stimulation
  • Self-assembling process
  • Tissue engineering

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Medicine(all)

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