Effects of co-cultures of meniscus cells and articular chondrocytes on PLLA scaffolds

Najmuddin J. Gunja, Kyriacos A. Athanasiou

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


The knee meniscus, a fibrocartilaginous tissue located in the knee joint, is characterized by heterogeneity in extracellular matrix and biomechanical properties. To recreate these properties using a tissue engineering approach, co-cultures of meniscus cells (MCs) and articular chondrocytes (ACs) were seeded in varying ratios (100:0, 75:25, 50:50, 25:75, and 0:100) on poly-L-lactic acid (PLLA) scaffolds and cultured in serum-free medium for 4 weeks. Histological, biochemical, and biomechanical tests were used to assess constructs at the end time point. Strong staining for collagen and glycosaminoglycan (GAG) was observed in all groups. Constructs with 100% MCs were positive for collagen I and constructs cultured with 100% ACs were positive for collagen II, while a mixture of collagen I and II was observed in other co-culture groups. Total collagen and GAG per construct increased as the percentage of ACs increased (27±8 μg, 0% AC to 45±8 μg, 100% ACs for collagen and 12±4 μg, 0% ACs to 40±5 μg, 100% ACs for GAG). Compressive modulus (instantaneous and relaxation modulus) of the constructs was significantly higher in the 100% ACs group (63±12 and 22±9 kPa, respectively) when compared to groups with higher percentage of MCs. No differences in tensile properties were noted among groups. Specific co-culture ratios were identified mimicking the GAG/DW of the inner (0:100, 25:75, and 50:50) and outer regions (100:0) of the meniscus. Overall, it was demonstrated that co-culturing MCs and ACs on PLLA scaffolds results in functional tissue engineered meniscus constructs with a spectrum of biochemical and biomechanical properties.

Original languageEnglish (US)
Pages (from-to)808-816
Number of pages9
JournalBiotechnology and Bioengineering
Issue number4
StatePublished - Jul 1 2009
Externally publishedYes


  • Articular chondrocytes
  • Co-cultures
  • Meniscus cells
  • PLLA
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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