Clostridium perfringens exotoxins have been implicated as major virulence factors responsible fur shock and organ failure in gas gangrene, yet the mechanism(s) by which they mediate cardiovascular dysfunction remain enigmatic. Recombinant (r) phospholipase C (PLC), rθ-toxin, culture supernatant (crude toxin), or 0.9% NaCl was infused intravenously into awake rabbits. Cardiac index (CI), mean arterial pressure (MAP), heart rate (HR), central venous pressure (CVP), arterial blood gases, and hematocrit were measured 1 h before and for 3 h after toxin infusion. Crude toxin and rPLC decreased CI, MAP, and HR and increased CVP; mortality was 87.5% and 83%, respectively. rθ-toxin did not decrease CI or MAP and mortality was 25%. Further, crude toxin and rPLC but not rθ-toxin inhibited cardiac contractility (dF/dt) in isolated rabbit atrial muscles. These results suggest that PLC-induced myocardial dysfunction contributes to shock in C. perfringens infection.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Infectious Diseases|
|State||Published - 1995|
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health