Effects of an ambient level of ozone on primate nasal epithelial mucosubstances

Quantitative histochemistry

J. R. Harkema, Charles Plopper, D. M. Hyde, J. A. St George, D. L. Dungworth

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Despite the absorption of inhaled oxidant gases by the nasal cavity, little effort has been made to characterize the effects of these oxidants on the nasal mucosa. This study defines the effects of ambient concentrations of ozone on the character and amount of mucosubstances in epithelium of nasal mucosa. Bonnet monkeys were exposed to 0.00 or 0.15 ppm O3 (8 hr/day) for 6 or 90 days, anesthetized, and exsanguinated. Nasal cavities were fixed with Karnovsky's fixative, decalcified, and processed for light microscopy, and sections were stained with alcian blue (pH 2.5)/periodic acid-Schiff or high iron diamine. Volume densities of secretory material in nasal epithelium were determined with the use of a Quantimet 900 image analyzer. After 6 days' exposure there were significant increases in both acidic and neutral glycoconjugates stored in transitional and respiratory epithelium. After 90 days there was significantly less mucosubstance than at 6 days. Only in the transitional epithelium did the total and sulfated mucosubstance remain greater than that of controls. Nasopharyngeal epithelium was minimally affected after 6 days of O3 and unchanged after 90 days. It is concluded that exposures to ambient levels of O3 induce significant changes in the stored secretory product of nasal epithelium.

Original languageEnglish (US)
Pages (from-to)90-96
Number of pages7
JournalAmerican Journal of Pathology
Volume127
Issue number1
StatePublished - 1987

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Nasal Mucosa
Ozone
Nose
Primates
Epithelium
Nasal Cavity
Oxidants
Macaca radiata
Respiratory Mucosa
Alcian Blue
Fixatives
Periodic Acid
Glycoconjugates
Diamines
Microscopy
Iron
Gases
Light

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Effects of an ambient level of ozone on primate nasal epithelial mucosubstances : Quantitative histochemistry. / Harkema, J. R.; Plopper, Charles; Hyde, D. M.; St George, J. A.; Dungworth, D. L.

In: American Journal of Pathology, Vol. 127, No. 1, 1987, p. 90-96.

Research output: Contribution to journalArticle

Harkema, J. R. ; Plopper, Charles ; Hyde, D. M. ; St George, J. A. ; Dungworth, D. L. / Effects of an ambient level of ozone on primate nasal epithelial mucosubstances : Quantitative histochemistry. In: American Journal of Pathology. 1987 ; Vol. 127, No. 1. pp. 90-96.
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