Effects of aging on cytochrome B5 expression in the human adrenal gland

Sejal Dharia, Audry Slane, Ming Jian, Michael Conner, Alan J Conley, Robert M. Brissie, C. Richard Parker

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Context: Aging in humans is characterized by a selective decline in circulating levels of adrenal androgens. The results of in vivo studies are suggestive of reduced adrenal 17,20-lyase activity in aging men and women. Objective: We sought to determine whether there are changes in the distribution and/or expression of cytochrome B5 (CytB5), an accessory protein important in the regulation of 17,20-lyase activity, in the adrenals of aging humans. Design: Comparison between younger and older adrenal glands. Setting: The study was conducted in a University Center. Patients or Other Participants: Adrenal glands obtained at autopsy after sudden death as a result of trauma from 46 young (age 20-40 yr) and 26 older (age 50-91 yr) humans were obtained and fixed within 24 h postmortem. Interventions: Paraffin sections were stained with hematoxylin and eosin and also were subjected to immunohistochemical staining for CytB5. All sections were quantitatively evaluated using an image capture and analysis program and qualitatively evaluated with respect to staining intensity. Main Outcome Measures: To determine whether there are any changes in CytB5 distribution in the adult human adrenal cortex during the aging process using qualitative and quantitative analysis with respect to age, gender, race, and postmortem interval. Results: CytB5 immunoreactivity was found in cells that corresponded to those of the zona reticularis. The percentage of the adrenal cortex immunoreactive for CytB5 decreased with aging (38.6 ± 7.6% for young and 30.1 ± 5.9% for older, mean ± SD; P < 0.0001) as did the percentage of adrenocortical tissue comprising the zona reticularis (36.8 ± 10.8% for young and 27.2 ± 5.9% for older; P < 0.001). However, there was no apparent change in the staining intensity of CytB5 among those cells that were immunopositive for this factor with aging. Conclusions: There appears to be a reduction in the proportion of the adrenal cortex that expresses CytB5 with aging, and this likely corresponds to a shrinkage of the zona reticularis. The mechanism and cause for this cortical regression are unknown.

Original languageEnglish (US)
Pages (from-to)4357-4361
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume90
Issue number7
DOIs
StatePublished - Jul 2005

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Cytochromes b5
Adrenal Glands
Aging of materials
Zona Reticularis
Adrenal Cortex
Steroid 17-alpha-Hydroxylase
Staining and Labeling
Accessories
Hematoxylin
Eosine Yellowish-(YS)
Sudden Death
Paraffin
Androgens
Autopsy
Outcome Assessment (Health Care)
Tissue
Wounds and Injuries
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Effects of aging on cytochrome B5 expression in the human adrenal gland. / Dharia, Sejal; Slane, Audry; Jian, Ming; Conner, Michael; Conley, Alan J; Brissie, Robert M.; Parker, C. Richard.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 90, No. 7, 07.2005, p. 4357-4361.

Research output: Contribution to journalArticle

Dharia, Sejal ; Slane, Audry ; Jian, Ming ; Conner, Michael ; Conley, Alan J ; Brissie, Robert M. ; Parker, C. Richard. / Effects of aging on cytochrome B5 expression in the human adrenal gland. In: Journal of Clinical Endocrinology and Metabolism. 2005 ; Vol. 90, No. 7. pp. 4357-4361.
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abstract = "Context: Aging in humans is characterized by a selective decline in circulating levels of adrenal androgens. The results of in vivo studies are suggestive of reduced adrenal 17,20-lyase activity in aging men and women. Objective: We sought to determine whether there are changes in the distribution and/or expression of cytochrome B5 (CytB5), an accessory protein important in the regulation of 17,20-lyase activity, in the adrenals of aging humans. Design: Comparison between younger and older adrenal glands. Setting: The study was conducted in a University Center. Patients or Other Participants: Adrenal glands obtained at autopsy after sudden death as a result of trauma from 46 young (age 20-40 yr) and 26 older (age 50-91 yr) humans were obtained and fixed within 24 h postmortem. Interventions: Paraffin sections were stained with hematoxylin and eosin and also were subjected to immunohistochemical staining for CytB5. All sections were quantitatively evaluated using an image capture and analysis program and qualitatively evaluated with respect to staining intensity. Main Outcome Measures: To determine whether there are any changes in CytB5 distribution in the adult human adrenal cortex during the aging process using qualitative and quantitative analysis with respect to age, gender, race, and postmortem interval. Results: CytB5 immunoreactivity was found in cells that corresponded to those of the zona reticularis. The percentage of the adrenal cortex immunoreactive for CytB5 decreased with aging (38.6 ± 7.6{\%} for young and 30.1 ± 5.9{\%} for older, mean ± SD; P < 0.0001) as did the percentage of adrenocortical tissue comprising the zona reticularis (36.8 ± 10.8{\%} for young and 27.2 ± 5.9{\%} for older; P < 0.001). However, there was no apparent change in the staining intensity of CytB5 among those cells that were immunopositive for this factor with aging. Conclusions: There appears to be a reduction in the proportion of the adrenal cortex that expresses CytB5 with aging, and this likely corresponds to a shrinkage of the zona reticularis. The mechanism and cause for this cortical regression are unknown.",
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T1 - Effects of aging on cytochrome B5 expression in the human adrenal gland

AU - Dharia, Sejal

AU - Slane, Audry

AU - Jian, Ming

AU - Conner, Michael

AU - Conley, Alan J

AU - Brissie, Robert M.

AU - Parker, C. Richard

PY - 2005/7

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N2 - Context: Aging in humans is characterized by a selective decline in circulating levels of adrenal androgens. The results of in vivo studies are suggestive of reduced adrenal 17,20-lyase activity in aging men and women. Objective: We sought to determine whether there are changes in the distribution and/or expression of cytochrome B5 (CytB5), an accessory protein important in the regulation of 17,20-lyase activity, in the adrenals of aging humans. Design: Comparison between younger and older adrenal glands. Setting: The study was conducted in a University Center. Patients or Other Participants: Adrenal glands obtained at autopsy after sudden death as a result of trauma from 46 young (age 20-40 yr) and 26 older (age 50-91 yr) humans were obtained and fixed within 24 h postmortem. Interventions: Paraffin sections were stained with hematoxylin and eosin and also were subjected to immunohistochemical staining for CytB5. All sections were quantitatively evaluated using an image capture and analysis program and qualitatively evaluated with respect to staining intensity. Main Outcome Measures: To determine whether there are any changes in CytB5 distribution in the adult human adrenal cortex during the aging process using qualitative and quantitative analysis with respect to age, gender, race, and postmortem interval. Results: CytB5 immunoreactivity was found in cells that corresponded to those of the zona reticularis. The percentage of the adrenal cortex immunoreactive for CytB5 decreased with aging (38.6 ± 7.6% for young and 30.1 ± 5.9% for older, mean ± SD; P < 0.0001) as did the percentage of adrenocortical tissue comprising the zona reticularis (36.8 ± 10.8% for young and 27.2 ± 5.9% for older; P < 0.001). However, there was no apparent change in the staining intensity of CytB5 among those cells that were immunopositive for this factor with aging. Conclusions: There appears to be a reduction in the proportion of the adrenal cortex that expresses CytB5 with aging, and this likely corresponds to a shrinkage of the zona reticularis. The mechanism and cause for this cortical regression are unknown.

AB - Context: Aging in humans is characterized by a selective decline in circulating levels of adrenal androgens. The results of in vivo studies are suggestive of reduced adrenal 17,20-lyase activity in aging men and women. Objective: We sought to determine whether there are changes in the distribution and/or expression of cytochrome B5 (CytB5), an accessory protein important in the regulation of 17,20-lyase activity, in the adrenals of aging humans. Design: Comparison between younger and older adrenal glands. Setting: The study was conducted in a University Center. Patients or Other Participants: Adrenal glands obtained at autopsy after sudden death as a result of trauma from 46 young (age 20-40 yr) and 26 older (age 50-91 yr) humans were obtained and fixed within 24 h postmortem. Interventions: Paraffin sections were stained with hematoxylin and eosin and also were subjected to immunohistochemical staining for CytB5. All sections were quantitatively evaluated using an image capture and analysis program and qualitatively evaluated with respect to staining intensity. Main Outcome Measures: To determine whether there are any changes in CytB5 distribution in the adult human adrenal cortex during the aging process using qualitative and quantitative analysis with respect to age, gender, race, and postmortem interval. Results: CytB5 immunoreactivity was found in cells that corresponded to those of the zona reticularis. The percentage of the adrenal cortex immunoreactive for CytB5 decreased with aging (38.6 ± 7.6% for young and 30.1 ± 5.9% for older, mean ± SD; P < 0.0001) as did the percentage of adrenocortical tissue comprising the zona reticularis (36.8 ± 10.8% for young and 27.2 ± 5.9% for older; P < 0.001). However, there was no apparent change in the staining intensity of CytB5 among those cells that were immunopositive for this factor with aging. Conclusions: There appears to be a reduction in the proportion of the adrenal cortex that expresses CytB5 with aging, and this likely corresponds to a shrinkage of the zona reticularis. The mechanism and cause for this cortical regression are unknown.

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