Effects of Aerobic and Anaerobic Fluid Collection on Biochemical Analysis of Peritoneal Fluid in Healthy Horses and Horses with Colic

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Objective: To determine whether in healthy horses and those with colic, exposure of peritoneal fluid to room air affects values obtained on biochemical analysis.Study Design: Prospective study.Animals: Adult horses with a primary complaint of acute abdominal pain (n=29) and 12 healthy horses.Methods: Peritoneal fluid was aseptically collected under aerobic and anaerobic conditions. After collection, pH, PCO2, PO2, HCO3 -, Na+, ionized Ca2+, K+, lactate, and glucose were immediately measured using a commercial blood gas analyzer. Biochemical variables were compared between aerobically and anaerobically obtained samples using a paired t-test.Results: In healthy horses, peritoneal fluid samples collected under anaerobic conditions had higher PCO2 and ionized Ca2+ and lower PO2, HCO3 -, and pH compared with samples exposed to air. No differences were observed for K+, Na+, glucose, and lactate. In horses with colic, samples collected anaerobically had higher PCO2, ionized Ca2+, Na+, and glucose and lower PO2, HCO3 -, and pH value compared with samples exposed to air. No differences were observed for K+ and lactate.Conclusion: Exposure of peritoneal fluid to room air had a significant effect on pH, PCO2, PO2, and variables associated or dependent on changes in pH such as HCO3 - and ionized Ca2+. Interpretation of biochemical analysis of peritoneal fluid may be influenced by sample collection method.

Original languageEnglish (US)
Pages (from-to)40-45
Number of pages6
JournalVeterinary Surgery
Volume40
Issue number1
DOIs
StatePublished - Jan 2011

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Effects of Aerobic and Anaerobic Fluid Collection on Biochemical Analysis of Peritoneal Fluid in Healthy Horses and Horses with Colic'. Together they form a unique fingerprint.

  • Cite this