Abstract
The in vivo effect of adenine on xanthine dehydrogenase from Drosophila melanogaster has been studied by feeding adult flies for 48 hr on regular undefined media buffered with phosphate and containing 15 mM adenine. Extracts of adenine-fed flies show 25-30% reduction in xanthine dehydrogenase activity, and 35-40% reduction in uric acid production. The adenine exposed enzyme, however, is now more stable during overnight dialysis with the loss of activity never more than 30%, while controls show losses of over 50%. Thermal stability studies reveal that the enzyme from adenine-fed flies, in contrast to that from controls, is resistant to high temperature (50°C). During the 48-hr period of feeding, enzyme activity decreases in the control flies whereas this ageing effect is less pronounced in the adenine-fed flies. Column chromatography and gel electrophoresis, using [8-14C]adenine show that adenine appears to bind to xanthine dehydrogenase, with the radioactivity peaks always corresponding with enzyme activity peaks.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 307-311 |
| Number of pages | 5 |
| Journal | Insect Biochemistry |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1986 |
| Externally published | Yes |
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Keywords
- adenine
- Drosophila melanogaster
- enzyme stability
- Xanthine dehydrogenase
Cite this
Effects of adenine on the stability and expression of xanthine dehydrogenase from Drosophila melanogaster. / Hagopian, Kevork; Duke, E. J.
In: Insect Biochemistry, Vol. 16, No. 2, 01.01.1986, p. 307-311.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of adenine on the stability and expression of xanthine dehydrogenase from Drosophila melanogaster
AU - Hagopian, Kevork
AU - Duke, E. J.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - The in vivo effect of adenine on xanthine dehydrogenase from Drosophila melanogaster has been studied by feeding adult flies for 48 hr on regular undefined media buffered with phosphate and containing 15 mM adenine. Extracts of adenine-fed flies show 25-30% reduction in xanthine dehydrogenase activity, and 35-40% reduction in uric acid production. The adenine exposed enzyme, however, is now more stable during overnight dialysis with the loss of activity never more than 30%, while controls show losses of over 50%. Thermal stability studies reveal that the enzyme from adenine-fed flies, in contrast to that from controls, is resistant to high temperature (50°C). During the 48-hr period of feeding, enzyme activity decreases in the control flies whereas this ageing effect is less pronounced in the adenine-fed flies. Column chromatography and gel electrophoresis, using [8-14C]adenine show that adenine appears to bind to xanthine dehydrogenase, with the radioactivity peaks always corresponding with enzyme activity peaks.
AB - The in vivo effect of adenine on xanthine dehydrogenase from Drosophila melanogaster has been studied by feeding adult flies for 48 hr on regular undefined media buffered with phosphate and containing 15 mM adenine. Extracts of adenine-fed flies show 25-30% reduction in xanthine dehydrogenase activity, and 35-40% reduction in uric acid production. The adenine exposed enzyme, however, is now more stable during overnight dialysis with the loss of activity never more than 30%, while controls show losses of over 50%. Thermal stability studies reveal that the enzyme from adenine-fed flies, in contrast to that from controls, is resistant to high temperature (50°C). During the 48-hr period of feeding, enzyme activity decreases in the control flies whereas this ageing effect is less pronounced in the adenine-fed flies. Column chromatography and gel electrophoresis, using [8-14C]adenine show that adenine appears to bind to xanthine dehydrogenase, with the radioactivity peaks always corresponding with enzyme activity peaks.
KW - adenine
KW - Drosophila melanogaster
KW - enzyme stability
KW - Xanthine dehydrogenase
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UR - http://www.scopus.com/inward/citedby.url?scp=0345443822&partnerID=8YFLogxK
U2 - 10.1016/0020-1790(86)90040-5
DO - 10.1016/0020-1790(86)90040-5
M3 - Article
AN - SCOPUS:0345443822
VL - 16
SP - 307
EP - 311
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
SN - 0965-1748
IS - 2
ER -