Effects of adenine on the stability and expression of xanthine dehydrogenase from Drosophila virilis

Kevork Hagopian, E. J. Duke

Research output: Contribution to journalArticle


The properties of xanthine dehydrogenase from Drosophila virilis have been studied and the effects of adenine on the enzyme analysed. The enzyme has a much higher thermal stability than that from D. melanogaster. Ammonium sulphate fractionation of the extracts precipitated the enzyme in the 0-40% saturation stage whereas the D. melanogaster enzyme precipitates between 40-60% saturation. Xanthine dehydrogenase activity per mg protein in crude extracts is 40-45% lower in D. virilis than in D. melanogaster but it is more resistant to dialysis. In vitro addition of adenine inhibits both enzymes but does not appear to stabilise the D. virilis enzyme during dialysis.

Original languageEnglish (US)
Pages (from-to)457-461
Number of pages5
JournalInsect Biochemistry
Issue number5
StatePublished - Jan 1 1989
Externally publishedYes



  • adenine
  • Drosophila virilis
  • enzyme stability
  • xanthine dehydrogenase

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