Effects of a carotene-deficient diet on measures of oxidative susceptibility and superoxide dismutase activity in adult women

Zisca R. Dixon, Betty J. Burri, Andrew Clifford, Edwin N. Frankel, Barbara O. Schneeman, Elizabeth Parks, Nancy L. Keim, Teresa Barbieri, Mei Miau Wu, Alice K H Fong, Mary J. Kretsch, Anne L. Sowell, John W. Erdman

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The effect of consuming a low carotene diet (≈60 μg carotene/day) on oxidative susceptibility and superoxide dismutase (SOD) activity in women living in a metabolic research unit was evaluated. The diet had sufficient vitamins A, E, and C. The women ate the diet supplemented with 1500 μg/day β-carotene for 4 days (baseline), then the unsupplemented diet for 68 days (depletion), followed by the diet supplemented with > 15,000 μg/day carotene for 28 days (repletion). Production of hexanal, pentanal, and pentane by copper-oxidased plasma low density lipoproteins from carotene-depleted women was greater than their production of these compounds when repleted with carotene. Erythrocyte SOD activity was depressed in carotene-depleted women; it recovered with repletion. Thiobarbituric acid reactive substances in plasma of carotene-depleted women were elevated and diminished with repletion. Dietary carotene seems to be needed, not only as a precursor of vitamin A, but also to inhibit oxidative damage and decrease oxidation susceptibility.

Original languageEnglish (US)
Pages (from-to)537-544
Number of pages8
JournalFree Radical Biology and Medicine
Volume17
Issue number6
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Carotenoids
Nutrition
Superoxide Dismutase
Diet
Vitamin A
Plasmas
Thiobarbituric Acid Reactive Substances
Vitamin E
LDL Lipoproteins
Ascorbic Acid
Copper
Erythrocytes
Oxidation
Research

Keywords

  • Carotene
  • Free radicals
  • Human
  • Oxidative damage
  • Superoxide dismutase

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine(all)
  • Toxicology

Cite this

Effects of a carotene-deficient diet on measures of oxidative susceptibility and superoxide dismutase activity in adult women. / Dixon, Zisca R.; Burri, Betty J.; Clifford, Andrew; Frankel, Edwin N.; Schneeman, Barbara O.; Parks, Elizabeth; Keim, Nancy L.; Barbieri, Teresa; Wu, Mei Miau; Fong, Alice K H; Kretsch, Mary J.; Sowell, Anne L.; Erdman, John W.

In: Free Radical Biology and Medicine, Vol. 17, No. 6, 1994, p. 537-544.

Research output: Contribution to journalArticle

Dixon, ZR, Burri, BJ, Clifford, A, Frankel, EN, Schneeman, BO, Parks, E, Keim, NL, Barbieri, T, Wu, MM, Fong, AKH, Kretsch, MJ, Sowell, AL & Erdman, JW 1994, 'Effects of a carotene-deficient diet on measures of oxidative susceptibility and superoxide dismutase activity in adult women', Free Radical Biology and Medicine, vol. 17, no. 6, pp. 537-544. https://doi.org/10.1016/0891-5849(94)90093-0
Dixon, Zisca R. ; Burri, Betty J. ; Clifford, Andrew ; Frankel, Edwin N. ; Schneeman, Barbara O. ; Parks, Elizabeth ; Keim, Nancy L. ; Barbieri, Teresa ; Wu, Mei Miau ; Fong, Alice K H ; Kretsch, Mary J. ; Sowell, Anne L. ; Erdman, John W. / Effects of a carotene-deficient diet on measures of oxidative susceptibility and superoxide dismutase activity in adult women. In: Free Radical Biology and Medicine. 1994 ; Vol. 17, No. 6. pp. 537-544.
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