Effects of 5% sodium chloride ophthalmic ointment on thickness and morphology of the normal canine cornea

Michelle Samuel, Sara M Thomasy, Allison S. Calderon, Philip H Kass, Keith Collins, Christopher J Murphy

Research output: Contribution to journalArticle

Abstract

Objective: To determine the effect of 5% sodium chloride ophthalmic ointment (5% NaCl) on thickness and morphology of the normal canine cornea using ultrasonic pachymetry (USP), in vivo confocal microscopy (IVCM), and Fourier-domain optical coherence tomography (FD-OCT). Methods: Five healthy laboratory Beagles received ophthalmic examinations including USP, IVCM, and FD-OCT prior to and at fixed intervals following treatment. The right and left eyes were treated with 5% NaCl and artificial tears ophthalmic ointment (AT), respectively, every 2 hours for 4 treatments/d (days 2-9), and then hourly for 7 treatments/d (day 10). Treatment groups were statistically compared using mixed-effects linear regression. Results: Treatment with 5% NaCl resulted in a 12 μm decrease in corneal thickness from baseline (P <.001), while there was no significant difference in corneal thickness between values obtained at baseline and following treatment with AT (P =.82). Epithelial cell density significantly increased from baseline (530 ± 52 cells/mm 2 ) to 577 ± 43 and 567 ± 15 cells/mm 2 with 5% NaCl and AT, respectively (P =.003 and.005, respectively). However, keratocyte cell density in the anterior and posterior stroma and endothelial cell density did not significantly differ following treatment with 5% NaCl or AT ointment (P >.05). Conclusions: Short-term topical treatment with 5% NaCl decreased corneal thickness in normal dogs with no observable changes in corneal morphology or signs of ocular toxicity.

Original languageEnglish (US)
Pages (from-to)229-237
Number of pages9
JournalVeterinary Ophthalmology
Volume22
Issue number3
DOIs
StatePublished - May 1 2019

Fingerprint

ointments
cornea
Ointments
Sodium Chloride
sodium chloride
Cornea
Canidae
eyes
Cell Count
dogs
Optical Coherence Tomography
Confocal Microscopy
Ultrasonics
Artificial Eye
tomography
ultrasonics
Linear Models
Endothelial Cells
Epithelial Cells
Dogs

Keywords

  • 5% sodium chloride ophthalmic ointment
  • canine
  • corneal edema
  • in vivo confocal microscopy
  • optical coherence tomography
  • ultrasonic pachymetry

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Effects of 5% sodium chloride ophthalmic ointment on thickness and morphology of the normal canine cornea. / Samuel, Michelle; Thomasy, Sara M; Calderon, Allison S.; Kass, Philip H; Collins, Keith; Murphy, Christopher J.

In: Veterinary Ophthalmology, Vol. 22, No. 3, 01.05.2019, p. 229-237.

Research output: Contribution to journalArticle

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