Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons

Michael A Rogawski; Jeffery L. Barker

doi:10.1016/0006-8993(83)91190-3

Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons

Michael A Rogawski, Jeffery L. Barker

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

We examined the effects of 4-aminopyridine (4-AP) on calcium conductance mechanisms in cultured mouse spinal cord neurons. At low concentrations (≤ 1 mM), 4-AP enhanced Ca²⁺-dependent transmitter release and prolonged the duration of Ca²⁺-dependent action potentials. Voltage clamp studies indicated that 4-AP directly facilitates Ca²⁺ entry through voltage sensitive channels apart from an effect on K⁺ currents. These results may help to explain why the drug promotes Ca²⁺-dependent transmitter release at peripheral and central synapses.

Original language	English (US)
Pages (from-to)	180-185
Number of pages	6
Journal	Brain Research
Volume	280
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)91190-3
State	Published - Nov 28 1983
Externally published	Yes

Keywords

4-aminopyridine
neuronal cell culture
voltage clamp
voltage-dependent calcium channel

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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AU - Rogawski, Michael A

AU - Barker, Jeffery L.

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N2 - We examined the effects of 4-aminopyridine (4-AP) on calcium conductance mechanisms in cultured mouse spinal cord neurons. At low concentrations (≤ 1 mM), 4-AP enhanced Ca2+-dependent transmitter release and prolonged the duration of Ca2+-dependent action potentials. Voltage clamp studies indicated that 4-AP directly facilitates Ca2+ entry through voltage sensitive channels apart from an effect on K+ currents. These results may help to explain why the drug promotes Ca2+-dependent transmitter release at peripheral and central synapses.

AB - We examined the effects of 4-aminopyridine (4-AP) on calcium conductance mechanisms in cultured mouse spinal cord neurons. At low concentrations (≤ 1 mM), 4-AP enhanced Ca2+-dependent transmitter release and prolonged the duration of Ca2+-dependent action potentials. Voltage clamp studies indicated that 4-AP directly facilitates Ca2+ entry through voltage sensitive channels apart from an effect on K+ currents. These results may help to explain why the drug promotes Ca2+-dependent transmitter release at peripheral and central synapses.

KW - 4-aminopyridine

KW - neuronal cell culture

KW - voltage clamp

KW - voltage-dependent calcium channel

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Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons

Abstract

Keywords

ASJC Scopus subject areas

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