Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons

Michael A Rogawski; Jeffery L. Barker

doi:10.1016/0006-8993(83)91190-3

Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons

Michael A Rogawski, Jeffery L. Barker

Research output: Contribution to journal › Article

84 Citations (Scopus)

Abstract

We examined the effects of 4-aminopyridine (4-AP) on calcium conductance mechanisms in cultured mouse spinal cord neurons. At low concentrations (≤ 1 mM), 4-AP enhanced Ca²⁺-dependent transmitter release and prolonged the duration of Ca²⁺-dependent action potentials. Voltage clamp studies indicated that 4-AP directly facilitates Ca²⁺ entry through voltage sensitive channels apart from an effect on K⁺ currents. These results may help to explain why the drug promotes Ca²⁺-dependent transmitter release at peripheral and central synapses.

Original language	English (US)
Pages (from-to)	180-185
Number of pages	6
Journal	Brain Research
Volume	280
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)91190-3
State	Published - Nov 28 1983
Externally published	Yes

Fingerprint

4-Aminopyridine

Action Potentials

Calcium

Neurons

Synapses

Spinal Cord

Pharmaceutical Preparations

Keywords

4-aminopyridine
neuronal cell culture
voltage clamp
voltage-dependent calcium channel

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

Cite this

Rogawski, M. A., & Barker, J. L. (1983). Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons. Brain Research, 280(1), 180-185. https://doi.org/10.1016/0006-8993(83)91190-3

Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons. / Rogawski, Michael A; Barker, Jeffery L.

In: Brain Research, Vol. 280, No. 1, 28.11.1983, p. 180-185.

Research output: Contribution to journal › Article

Rogawski, MA & Barker, JL 1983, 'Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons', Brain Research, vol. 280, no. 1, pp. 180-185. https://doi.org/10.1016/0006-8993(83)91190-3

Rogawski MA, Barker JL. Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons. Brain Research. 1983 Nov 28;280(1):180-185. https://doi.org/10.1016/0006-8993(83)91190-3

Rogawski, Michael A ; Barker, Jeffery L. / Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons. In: Brain Research. 1983 ; Vol. 280, No. 1. pp. 180-185.

@article{6b0a04caaa5b48a5a361f90c7be7950d,

title = "Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons",

abstract = "We examined the effects of 4-aminopyridine (4-AP) on calcium conductance mechanisms in cultured mouse spinal cord neurons. At low concentrations (≤ 1 mM), 4-AP enhanced Ca2+-dependent transmitter release and prolonged the duration of Ca2+-dependent action potentials. Voltage clamp studies indicated that 4-AP directly facilitates Ca2+ entry through voltage sensitive channels apart from an effect on K+ currents. These results may help to explain why the drug promotes Ca2+-dependent transmitter release at peripheral and central synapses.",

keywords = "4-aminopyridine, neuronal cell culture, voltage clamp, voltage-dependent calcium channel",

author = "Rogawski, {Michael A} and Barker, {Jeffery L.}",

year = "1983",

month = "11",

day = "28",

doi = "10.1016/0006-8993(83)91190-3",

language = "English (US)",

volume = "280",

pages = "180--185",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Effects of 4-aminopyridine on calcium action potentials and calcium current under voltage clamp in spinal neurons

AU - Rogawski, Michael A

AU - Barker, Jeffery L.

PY - 1983/11/28

Y1 - 1983/11/28

N2 - We examined the effects of 4-aminopyridine (4-AP) on calcium conductance mechanisms in cultured mouse spinal cord neurons. At low concentrations (≤ 1 mM), 4-AP enhanced Ca2+-dependent transmitter release and prolonged the duration of Ca2+-dependent action potentials. Voltage clamp studies indicated that 4-AP directly facilitates Ca2+ entry through voltage sensitive channels apart from an effect on K+ currents. These results may help to explain why the drug promotes Ca2+-dependent transmitter release at peripheral and central synapses.

AB - We examined the effects of 4-aminopyridine (4-AP) on calcium conductance mechanisms in cultured mouse spinal cord neurons. At low concentrations (≤ 1 mM), 4-AP enhanced Ca2+-dependent transmitter release and prolonged the duration of Ca2+-dependent action potentials. Voltage clamp studies indicated that 4-AP directly facilitates Ca2+ entry through voltage sensitive channels apart from an effect on K+ currents. These results may help to explain why the drug promotes Ca2+-dependent transmitter release at peripheral and central synapses.

KW - 4-aminopyridine

KW - neuronal cell culture

KW - voltage clamp

KW - voltage-dependent calcium channel

UR - http://www.scopus.com/inward/record.url?scp=0021048721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021048721&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(83)91190-3

DO - 10.1016/0006-8993(83)91190-3

M3 - Article

C2 - 6652477

AN - SCOPUS:0021048721

VL - 280

SP - 180

EP - 185

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -

Access to Document

10.1016/0006-8993(83)91190-3