Effects of 20 months of ozone exposure on lung collagen in Fischer 344 rats

Jerold A Last, Tom Gelzleichter, Jack Harkema, William C. Parks, Paul Mellick

Research output: Contribution to journalArticle

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Abstract

Fischer 344 rats were exposed to filtered air (controls) or to 0.12, 0.5, or 1.0 ppm of ozone for 6 h/day, 5 days/week, for 20 months. We examined lung collagen deposition and metabolism in tissue from these animals to determine whether chronic exposure of rats to ozone causes pulmonary fibrosis. We observed excess stainable collagen in the centriacinar region of lungs from the rats exposed to 0.5 or 1.0 ppm of ozone. Biochemical analysis indicated a slight, yet significant, excess collagen deposition in the female rats exposed to 0.5 or 1.0 ppm of ozone. Collagen in the lungs of the females also contained relatively more hydroxylysine-derived crosslinks than did lung collagen from age-matched control animals. No excess of type I procollagen mRNA could be appreciated by in situ hybridization in lungs of the rats exposed to 1.0 ppm of ozone for 20 months, although this mRNA was detected in occasional alveolar interstitial cells at 2 months of exposure to ozone under the same protocol. These findings indicate that chronic exposure of rats to ozone causes mild, persistent fibrosis. The significance of these observations with regard to human health risks of chronically inhaling ozone at ambient levels in polluted air remains to be determined.

Original languageEnglish (US)
Pages (from-to)83-102
Number of pages20
JournalToxicology
Volume84
Issue number1-3
DOIs
StatePublished - Nov 12 1993

Fingerprint

Ozone
Inbred F344 Rats
Rats
Collagen
Lung
Animals
Air
Hydroxylysine
Alveolar Epithelial Cells
Messenger RNA
Pulmonary Fibrosis
Health risks
Collagen Type I
Metabolism
Inhalation
In Situ Hybridization
Fibrosis
Tissue
Health

Keywords

  • Air pollution
  • Collagen
  • Lung
  • Ozone
  • Pulmonary fibrosis

ASJC Scopus subject areas

  • Toxicology

Cite this

Effects of 20 months of ozone exposure on lung collagen in Fischer 344 rats. / Last, Jerold A; Gelzleichter, Tom; Harkema, Jack; Parks, William C.; Mellick, Paul.

In: Toxicology, Vol. 84, No. 1-3, 12.11.1993, p. 83-102.

Research output: Contribution to journalArticle

Last, JA, Gelzleichter, T, Harkema, J, Parks, WC & Mellick, P 1993, 'Effects of 20 months of ozone exposure on lung collagen in Fischer 344 rats', Toxicology, vol. 84, no. 1-3, pp. 83-102. https://doi.org/10.1016/0300-483X(93)90110-E
Last, Jerold A ; Gelzleichter, Tom ; Harkema, Jack ; Parks, William C. ; Mellick, Paul. / Effects of 20 months of ozone exposure on lung collagen in Fischer 344 rats. In: Toxicology. 1993 ; Vol. 84, No. 1-3. pp. 83-102.
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