Abstract
1. The parthenogenetic weevil Otiorhynchus sulcatus was collected from five geographical locations in the U.S.A. All weevils from each location were infected by Wolbachia belonging to supergroup B. 2. We hypothesized that treatment with tetracycline, which has been used to clear Wolbachia infection, would influence the reproduction of Wolbachia-infected parthenogenetic O. sulcatus females. Tetracycline treatment of preovipositional O. sulcatus females specifically inhibited egg hatching but had no effect relative to controls on any other physiological trait. 3. Treatment with gentamicin, which reportedly has no effect on Wolbachia infection but is indistinguishable from tetracycline in inhibiting protein synthesis of other bacterial genera, did not influence egg hatching. 4. These findings strongly suggest that the inhibition of egg development results from the antibiotic effect on Wolbachia rather than by direct toxic effects on O. sulcatus physiology. 5. We speculate that Wolbachia may be necessary for normal development of O. sulcatus eggs and discuss the implications of these findings for O. sulcatus ecology.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 147-155 |
| Number of pages | 9 |
| Journal | Agricultural and Forest Entomology |
| Volume | 10 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 2008 |
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Keywords
- Coleoptera
- Curculionidae
- Egg hatching
- Gentamicin
- Otiorhynchus sulcatus
- Tetracycline
- Wolbachia
ASJC Scopus subject areas
- Plant Science
- Insect Science
- Agronomy and Crop Science
- Animal Science and Zoology
Cite this
Effects and implications of antibiotic treatment on Wolbachia-infected vine weevil (Coleoptera : Curculionidae). / Son, Youngsoo; Luckhart, Shirley; Zhang, Xing; Lieber, Matthew J.; Lewis, Edwin E.
In: Agricultural and Forest Entomology, Vol. 10, No. 2, 05.2008, p. 147-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects and implications of antibiotic treatment on Wolbachia-infected vine weevil (Coleoptera
T2 - Curculionidae)
AU - Son, Youngsoo
AU - Luckhart, Shirley
AU - Zhang, Xing
AU - Lieber, Matthew J.
AU - Lewis, Edwin E.
PY - 2008/5
Y1 - 2008/5
N2 - 1. The parthenogenetic weevil Otiorhynchus sulcatus was collected from five geographical locations in the U.S.A. All weevils from each location were infected by Wolbachia belonging to supergroup B. 2. We hypothesized that treatment with tetracycline, which has been used to clear Wolbachia infection, would influence the reproduction of Wolbachia-infected parthenogenetic O. sulcatus females. Tetracycline treatment of preovipositional O. sulcatus females specifically inhibited egg hatching but had no effect relative to controls on any other physiological trait. 3. Treatment with gentamicin, which reportedly has no effect on Wolbachia infection but is indistinguishable from tetracycline in inhibiting protein synthesis of other bacterial genera, did not influence egg hatching. 4. These findings strongly suggest that the inhibition of egg development results from the antibiotic effect on Wolbachia rather than by direct toxic effects on O. sulcatus physiology. 5. We speculate that Wolbachia may be necessary for normal development of O. sulcatus eggs and discuss the implications of these findings for O. sulcatus ecology.
AB - 1. The parthenogenetic weevil Otiorhynchus sulcatus was collected from five geographical locations in the U.S.A. All weevils from each location were infected by Wolbachia belonging to supergroup B. 2. We hypothesized that treatment with tetracycline, which has been used to clear Wolbachia infection, would influence the reproduction of Wolbachia-infected parthenogenetic O. sulcatus females. Tetracycline treatment of preovipositional O. sulcatus females specifically inhibited egg hatching but had no effect relative to controls on any other physiological trait. 3. Treatment with gentamicin, which reportedly has no effect on Wolbachia infection but is indistinguishable from tetracycline in inhibiting protein synthesis of other bacterial genera, did not influence egg hatching. 4. These findings strongly suggest that the inhibition of egg development results from the antibiotic effect on Wolbachia rather than by direct toxic effects on O. sulcatus physiology. 5. We speculate that Wolbachia may be necessary for normal development of O. sulcatus eggs and discuss the implications of these findings for O. sulcatus ecology.
KW - Coleoptera
KW - Curculionidae
KW - Egg hatching
KW - Gentamicin
KW - Otiorhynchus sulcatus
KW - Tetracycline
KW - Wolbachia
UR - http://www.scopus.com/inward/record.url?scp=42149170669&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149170669&partnerID=8YFLogxK
U2 - 10.1111/j.1461-9563.2008.00369.x
DO - 10.1111/j.1461-9563.2008.00369.x
M3 - Article
AN - SCOPUS:42149170669
VL - 10
SP - 147
EP - 155
JO - Agricultural and Forest Entomology
JF - Agricultural and Forest Entomology
SN - 1461-9555
IS - 2
ER -