Effects and implications of antibiotic treatment on Wolbachia-infected vine weevil (Coleoptera: Curculionidae)

Youngsoo Son, Shirley Luckhart, Xing Zhang, Matthew J. Lieber, Edwin E. Lewis

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

1. The parthenogenetic weevil Otiorhynchus sulcatus was collected from five geographical locations in the U.S.A. All weevils from each location were infected by Wolbachia belonging to supergroup B. 2. We hypothesized that treatment with tetracycline, which has been used to clear Wolbachia infection, would influence the reproduction of Wolbachia-infected parthenogenetic O. sulcatus females. Tetracycline treatment of preovipositional O. sulcatus females specifically inhibited egg hatching but had no effect relative to controls on any other physiological trait. 3. Treatment with gentamicin, which reportedly has no effect on Wolbachia infection but is indistinguishable from tetracycline in inhibiting protein synthesis of other bacterial genera, did not influence egg hatching. 4. These findings strongly suggest that the inhibition of egg development results from the antibiotic effect on Wolbachia rather than by direct toxic effects on O. sulcatus physiology. 5. We speculate that Wolbachia may be necessary for normal development of O. sulcatus eggs and discuss the implications of these findings for O. sulcatus ecology.

Original languageEnglish (US)
Pages (from-to)147-155
Number of pages9
JournalAgricultural and Forest Entomology
Volume10
Issue number2
DOIs
StatePublished - May 2008

Fingerprint

Otiorhynchus sulcatus
Wolbachia
vine
antibiotics
Curculionidae
vines
egg
Coleoptera
hatching
tetracycline
egg development
physiology
ecology
protein
Geographical Locations
gentamicin
infection
effect
protein synthesis

Keywords

  • Coleoptera
  • Curculionidae
  • Egg hatching
  • Gentamicin
  • Otiorhynchus sulcatus
  • Tetracycline
  • Wolbachia

ASJC Scopus subject areas

  • Plant Science
  • Insect Science
  • Agronomy and Crop Science
  • Animal Science and Zoology

Cite this

Effects and implications of antibiotic treatment on Wolbachia-infected vine weevil (Coleoptera : Curculionidae). / Son, Youngsoo; Luckhart, Shirley; Zhang, Xing; Lieber, Matthew J.; Lewis, Edwin E.

In: Agricultural and Forest Entomology, Vol. 10, No. 2, 05.2008, p. 147-155.

Research output: Contribution to journalArticle

Son, Youngsoo ; Luckhart, Shirley ; Zhang, Xing ; Lieber, Matthew J. ; Lewis, Edwin E. / Effects and implications of antibiotic treatment on Wolbachia-infected vine weevil (Coleoptera : Curculionidae). In: Agricultural and Forest Entomology. 2008 ; Vol. 10, No. 2. pp. 147-155.
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