Effective one-pot multienzyme (OPME) synthesis of monotreme milk oligosaccharides and other sialosides containing 4-: O -acetyl sialic acid

Hai Yu, Jie Zeng, Yanhong Li, Vireak Thon, Baojun Shi, Xi Chen

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A facile one-pot two-enzyme chemoenzymatic approach has been established for the gram (Neu4,5Ac2α3Lac, 1.33 g) and preparative scale (Neu4,5Ac2α3LNnT) synthesis of monotreme milk oligosaccharides. Other O-acetyl-5-N-acetylneuraminic acid (Neu4,5Ac2)- or 4-O-acetyl-5-N-glycolylneuraminic acid (Neu4Ac5Gc) -containing α2-3-sialosides have also been synthesized in the preparative scale. Used as an effective probe, Neu4,5Ac2α3GalβpNP was found to be a suitable substrate by human influenza A viruses but not bacterial sialidases.

Original languageEnglish (US)
Pages (from-to)8586-8597
Number of pages12
JournalOrganic and Biomolecular Chemistry
Volume14
Issue number36
DOIs
StatePublished - 2016

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Effective one-pot multienzyme (OPME) synthesis of monotreme milk oligosaccharides and other sialosides containing 4-: O -acetyl sialic acid'. Together they form a unique fingerprint.

Cite this