Abstract
English walnuts are implicated in severe, IgE-mediated food allergy in humans. We sought to determine if polyphenolic compounds extracted from the edible nut could promote IgE production to a coadministered allergen. BALB/c mice were sensitized to ovalbumin (OVA) with or without alum (AL) or polyphenolic-enriched extract via intraperitoneal injection. Serum was analyzed for total IgE and OVA-specific IgE, IgG1, and IgG2a/2b. Coadministration of walnut polyphenolic-enriched extract with antigen and AL increased serum concentrations of antigen-specific IgE and IgG1. When AL was excluded from the injections, polyphenolic extract tended to enhance OVA-specific IgE and IgG1 over levels induced by OVA alone, but the increase did not reach significance. Serum IgG2a/2b levels were similar between mice receiving OVA/AL and OVA/AL with polyphenolics. Thus, walnut polyphenolic extract enhanced the Th2-skewing effect of an aluminum hydroxide adjuvant. This indicates that walnut polyphenolic compounds may play a role in allergic sensitization of genetically predisposed individuals.
| Original language | English (US) |
|---|---|
| Title of host publication | Annals of the New York Academy of Sciences |
| Pages | 58-69 |
| Number of pages | 12 |
| Volume | 1190 |
| DOIs | |
| State | Published - Mar 2010 |
Publication series
| Name | Annals of the New York Academy of Sciences |
|---|---|
| Volume | 1190 |
| ISSN (Print) | 00778923 |
| ISSN (Electronic) | 17496632 |
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Keywords
- Allergy
- Antibodies
- Food allergy
- Food matrix
- IgE
- Juglans
- Mouse
- Polyphenol
- Sensitization
- Walnut
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Effect of walnut (Juglans regia) polyphenolic compounds on ovalbumin-specific IgE induction in female BALB/c mice. / Comstock, Sarah S.; Gershwin, Laurel J; Teuber, Suzanne S.
Annals of the New York Academy of Sciences. Vol. 1190 2010. p. 58-69 (Annals of the New York Academy of Sciences; Vol. 1190).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Effect of walnut (Juglans regia) polyphenolic compounds on ovalbumin-specific IgE induction in female BALB/c mice
AU - Comstock, Sarah S.
AU - Gershwin, Laurel J
AU - Teuber, Suzanne S
PY - 2010/3
Y1 - 2010/3
N2 - English walnuts are implicated in severe, IgE-mediated food allergy in humans. We sought to determine if polyphenolic compounds extracted from the edible nut could promote IgE production to a coadministered allergen. BALB/c mice were sensitized to ovalbumin (OVA) with or without alum (AL) or polyphenolic-enriched extract via intraperitoneal injection. Serum was analyzed for total IgE and OVA-specific IgE, IgG1, and IgG2a/2b. Coadministration of walnut polyphenolic-enriched extract with antigen and AL increased serum concentrations of antigen-specific IgE and IgG1. When AL was excluded from the injections, polyphenolic extract tended to enhance OVA-specific IgE and IgG1 over levels induced by OVA alone, but the increase did not reach significance. Serum IgG2a/2b levels were similar between mice receiving OVA/AL and OVA/AL with polyphenolics. Thus, walnut polyphenolic extract enhanced the Th2-skewing effect of an aluminum hydroxide adjuvant. This indicates that walnut polyphenolic compounds may play a role in allergic sensitization of genetically predisposed individuals.
AB - English walnuts are implicated in severe, IgE-mediated food allergy in humans. We sought to determine if polyphenolic compounds extracted from the edible nut could promote IgE production to a coadministered allergen. BALB/c mice were sensitized to ovalbumin (OVA) with or without alum (AL) or polyphenolic-enriched extract via intraperitoneal injection. Serum was analyzed for total IgE and OVA-specific IgE, IgG1, and IgG2a/2b. Coadministration of walnut polyphenolic-enriched extract with antigen and AL increased serum concentrations of antigen-specific IgE and IgG1. When AL was excluded from the injections, polyphenolic extract tended to enhance OVA-specific IgE and IgG1 over levels induced by OVA alone, but the increase did not reach significance. Serum IgG2a/2b levels were similar between mice receiving OVA/AL and OVA/AL with polyphenolics. Thus, walnut polyphenolic extract enhanced the Th2-skewing effect of an aluminum hydroxide adjuvant. This indicates that walnut polyphenolic compounds may play a role in allergic sensitization of genetically predisposed individuals.
KW - Allergy
KW - Antibodies
KW - Food allergy
KW - Food matrix
KW - IgE
KW - Juglans
KW - Mouse
KW - Polyphenol
KW - Sensitization
KW - Walnut
UR - http://www.scopus.com/inward/record.url?scp=77950255882&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950255882&partnerID=8YFLogxK
U2 - 10.1111/j.1749-6632.2009.05274.x
DO - 10.1111/j.1749-6632.2009.05274.x
M3 - Conference contribution
C2 - 20388137
AN - SCOPUS:77950255882
SN - 9781573317900
VL - 1190
T3 - Annals of the New York Academy of Sciences
SP - 58
EP - 69
BT - Annals of the New York Academy of Sciences
ER -