Effect of vitamin E on reoxygenation injury experienced by isolated rat hepatocytes

Paolo Caraceni, Tony Yao, Silvia Degli Esposti, Antonio Gasbarrini, Brian T. Bowie, Mark A Zern, Andre B. Borle, David H. Van Thiel

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The pathogenic role of lipid peroxidation in the reperfusion injury of the liver is still controversial. This study was performed to determine whether the damage caused by oxygen free radicals during reoxygenation in perfused rat hepatocytes is related to lipid peroxidation. Superoxide anion was detected by lucigenin-enhanced chemiluminescence. Lipid peroxidation and cell injury were assessed by the release of malondialdehyde and lactic dehydrogenase. Upon reoxygenation following 2.5 h of anoxia, isolated hepatocytes generated considerable amount of O2-. Following O2- formation, a significant increase in malondialdehyde release was measured. Cell injury was temporally delayed relative to O2- generation, but preceded the occurence of a significant lipid peroxidation. Treatment with Vitamin E abolished lipid peroxidation but had no effect upon superoxide anion formation and cell injury. These results suggest that in perfused rat hepatocytes non-peroxidative mechanisms are more important than peroxidative mechanisms in the pathogenesis of the early phases of reoxygenation injury.

Original languageEnglish (US)
Pages (from-to)1427-1432
Number of pages6
JournalLife Sciences
Volume55
Issue number18
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • lipid peroxidation
  • oxygen free radicals
  • reoxygenation injury
  • vitamin E

ASJC Scopus subject areas

  • Pharmacology

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