Abstract
The pathogenic role of lipid peroxidation in the reperfusion injury of the liver is still controversial. This study was performed to determine whether the damage caused by oxygen free radicals during reoxygenation in perfused rat hepatocytes is related to lipid peroxidation. Superoxide anion was detected by lucigenin-enhanced chemiluminescence. Lipid peroxidation and cell injury were assessed by the release of malondialdehyde and lactic dehydrogenase. Upon reoxygenation following 2.5 h of anoxia, isolated hepatocytes generated considerable amount of O2-. Following O2- formation, a significant increase in malondialdehyde release was measured. Cell injury was temporally delayed relative to O2- generation, but preceded the occurence of a significant lipid peroxidation. Treatment with Vitamin E abolished lipid peroxidation but had no effect upon superoxide anion formation and cell injury. These results suggest that in perfused rat hepatocytes non-peroxidative mechanisms are more important than peroxidative mechanisms in the pathogenesis of the early phases of reoxygenation injury.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1427-1432 |
| Number of pages | 6 |
| Journal | Life Sciences |
| Volume | 55 |
| Issue number | 18 |
| DOIs | |
| State | Published - 1994 |
| Externally published | Yes |
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Keywords
- lipid peroxidation
- oxygen free radicals
- reoxygenation injury
- vitamin E
ASJC Scopus subject areas
- Pharmacology
Cite this
Effect of vitamin E on reoxygenation injury experienced by isolated rat hepatocytes. / Caraceni, Paolo; Yao, Tony; Esposti, Silvia Degli; Gasbarrini, Antonio; Bowie, Brian T.; Zern, Mark A; Borle, Andre B.; Van Thiel, David H.
In: Life Sciences, Vol. 55, No. 18, 1994, p. 1427-1432.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of vitamin E on reoxygenation injury experienced by isolated rat hepatocytes
AU - Caraceni, Paolo
AU - Yao, Tony
AU - Esposti, Silvia Degli
AU - Gasbarrini, Antonio
AU - Bowie, Brian T.
AU - Zern, Mark A
AU - Borle, Andre B.
AU - Van Thiel, David H.
PY - 1994
Y1 - 1994
N2 - The pathogenic role of lipid peroxidation in the reperfusion injury of the liver is still controversial. This study was performed to determine whether the damage caused by oxygen free radicals during reoxygenation in perfused rat hepatocytes is related to lipid peroxidation. Superoxide anion was detected by lucigenin-enhanced chemiluminescence. Lipid peroxidation and cell injury were assessed by the release of malondialdehyde and lactic dehydrogenase. Upon reoxygenation following 2.5 h of anoxia, isolated hepatocytes generated considerable amount of O2-. Following O2- formation, a significant increase in malondialdehyde release was measured. Cell injury was temporally delayed relative to O2- generation, but preceded the occurence of a significant lipid peroxidation. Treatment with Vitamin E abolished lipid peroxidation but had no effect upon superoxide anion formation and cell injury. These results suggest that in perfused rat hepatocytes non-peroxidative mechanisms are more important than peroxidative mechanisms in the pathogenesis of the early phases of reoxygenation injury.
AB - The pathogenic role of lipid peroxidation in the reperfusion injury of the liver is still controversial. This study was performed to determine whether the damage caused by oxygen free radicals during reoxygenation in perfused rat hepatocytes is related to lipid peroxidation. Superoxide anion was detected by lucigenin-enhanced chemiluminescence. Lipid peroxidation and cell injury were assessed by the release of malondialdehyde and lactic dehydrogenase. Upon reoxygenation following 2.5 h of anoxia, isolated hepatocytes generated considerable amount of O2-. Following O2- formation, a significant increase in malondialdehyde release was measured. Cell injury was temporally delayed relative to O2- generation, but preceded the occurence of a significant lipid peroxidation. Treatment with Vitamin E abolished lipid peroxidation but had no effect upon superoxide anion formation and cell injury. These results suggest that in perfused rat hepatocytes non-peroxidative mechanisms are more important than peroxidative mechanisms in the pathogenesis of the early phases of reoxygenation injury.
KW - lipid peroxidation
KW - oxygen free radicals
KW - reoxygenation injury
KW - vitamin E
UR - http://www.scopus.com/inward/record.url?scp=0027956101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027956101&partnerID=8YFLogxK
U2 - 10.1016/0024-3205(94)00757-8
DO - 10.1016/0024-3205(94)00757-8
M3 - Article
C2 - 7934654
AN - SCOPUS:0027956101
VL - 55
SP - 1427
EP - 1432
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 18
ER -