Effect of vasoactive intestinal peptide on ion transport across dog tracheal epithelium

I. Nathanson, Jonathan Widdicombe, P. J. Barnes

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Under short-circuit conditions, vasoactive intestinal peptides (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (K(m) ≃ 10-8 M). The increase in Cl- secretion was not different from the rise in short-circuit current (I(sc)). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10-6 M) to VIP-treated tissues further increased the I(sc) by 54%. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10-5 or 10-6 M) or tetrodotoxin (10-6 M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.

Original languageEnglish (US)
Pages (from-to)1844-1848
Number of pages5
JournalJournal of Applied Physiology Respiratory Environmental and Exercise Physiology
Volume55
Issue number6
StatePublished - 1983
Externally publishedYes

Fingerprint

Vasoactive Intestinal Peptide
Ion Transport
Epithelium
Dogs
Phentolamine
Tetrodotoxin
Atropine
Isoproterenol
Propranolol
Epithelial Cells

ASJC Scopus subject areas

  • Endocrinology
  • Physiology

Cite this

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abstract = "Under short-circuit conditions, vasoactive intestinal peptides (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (K(m) ≃ 10-8 M). The increase in Cl- secretion was not different from the rise in short-circuit current (I(sc)). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10-6 M) to VIP-treated tissues further increased the I(sc) by 54{\%}. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10-5 or 10-6 M) or tetrodotoxin (10-6 M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.",
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T1 - Effect of vasoactive intestinal peptide on ion transport across dog tracheal epithelium

AU - Nathanson, I.

AU - Widdicombe, Jonathan

AU - Barnes, P. J.

PY - 1983

Y1 - 1983

N2 - Under short-circuit conditions, vasoactive intestinal peptides (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (K(m) ≃ 10-8 M). The increase in Cl- secretion was not different from the rise in short-circuit current (I(sc)). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10-6 M) to VIP-treated tissues further increased the I(sc) by 54%. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10-5 or 10-6 M) or tetrodotoxin (10-6 M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.

AB - Under short-circuit conditions, vasoactive intestinal peptides (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (K(m) ≃ 10-8 M). The increase in Cl- secretion was not different from the rise in short-circuit current (I(sc)). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10-6 M) to VIP-treated tissues further increased the I(sc) by 54%. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10-5 or 10-6 M) or tetrodotoxin (10-6 M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.

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