Abstract
Under short-circuit conditions, vasoactive intestinal peptides (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (K(m) ≃ 10-8 M). The increase in Cl- secretion was not different from the rise in short-circuit current (I(sc)). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10-6 M) to VIP-treated tissues further increased the I(sc) by 54%. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10-5 or 10-6 M) or tetrodotoxin (10-6 M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1844-1848 |
| Number of pages | 5 |
| Journal | Journal of Applied Physiology Respiratory Environmental and Exercise Physiology |
| Volume | 55 |
| Issue number | 6 |
| State | Published - 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Physiology
Cite this
Effect of vasoactive intestinal peptide on ion transport across dog tracheal epithelium. / Nathanson, I.; Widdicombe, Jonathan; Barnes, P. J.
In: Journal of Applied Physiology Respiratory Environmental and Exercise Physiology, Vol. 55, No. 6, 1983, p. 1844-1848.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of vasoactive intestinal peptide on ion transport across dog tracheal epithelium
AU - Nathanson, I.
AU - Widdicombe, Jonathan
AU - Barnes, P. J.
PY - 1983
Y1 - 1983
N2 - Under short-circuit conditions, vasoactive intestinal peptides (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (K(m) ≃ 10-8 M). The increase in Cl- secretion was not different from the rise in short-circuit current (I(sc)). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10-6 M) to VIP-treated tissues further increased the I(sc) by 54%. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10-5 or 10-6 M) or tetrodotoxin (10-6 M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.
AB - Under short-circuit conditions, vasoactive intestinal peptides (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (K(m) ≃ 10-8 M). The increase in Cl- secretion was not different from the rise in short-circuit current (I(sc)). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10-6 M) to VIP-treated tissues further increased the I(sc) by 54%. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10-5 or 10-6 M) or tetrodotoxin (10-6 M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021025254&partnerID=8YFLogxK
M3 - Article
C2 - 6662775
AN - SCOPUS:0021025254
VL - 55
SP - 1844
EP - 1848
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 6
ER -