Effect of vaginally administered (Ala8,13,18)-magainin II amide on the morphology of implantation stage endometrium in the rhesus monkey (Macaca mulatta)

D. Ghosh, Latika Dhawan, P. G L Lalitkumar, Viviana Wong, J. F. Rosario, Andrew G Hendrickx, B. L. Lasley, J. W. Overstreet, Jayasree Sengupta

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Intravaginal administration of an anti-microbial agent, (Ala8,13,18)-magainin II amide, during blastocyst implantation inhibits pregnancy establishment in a dose-related manner in the rhesus monkey (Macaca mulatta). In the present study, mated female rhesus monkeys were vaginally inserted with tampons containing vehicle (Group 1; n=5) and test agent (magainin, 0.5 mg/animal; Group 2; n=6) on cycle day 20. Endometrial tissue samples were collected on Cycle Day 24 from all monkeys and processed for morphometric and ultrastructural analysis. Concentrations of estradiol-17β, progesterone, and chorionic gonadotrophin in peripheral circulation were determined, which revealed that two monkeys in Group 1 were pregnant while no animals were pregnant in Group 2. Endometrial morphology, however, revealed histologic evidence of pregnancy in three out of the six magainin-treated animals. It appears that intra-vaginal administration of magainin II amide had a marginal effect on the implantation stage endometrium and the initiation of the implantation process in the rhesus monkey.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalContraception
Volume63
Issue number6
DOIs
StatePublished - 2001

Fingerprint

Magainins
Endometrium
Macaca mulatta
Amides
Intravaginal Administration
Haplorhini
Pregnancy
Chorionic Gonadotropin
Anti-Infective Agents
Progesterone
Estradiol

Keywords

  • Endometrium
  • Implantation
  • Magainin
  • Morphology
  • Rhesus monkey

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Effect of vaginally administered (Ala8,13,18)-magainin II amide on the morphology of implantation stage endometrium in the rhesus monkey (Macaca mulatta). / Ghosh, D.; Dhawan, Latika; Lalitkumar, P. G L; Wong, Viviana; Rosario, J. F.; Hendrickx, Andrew G; Lasley, B. L.; Overstreet, J. W.; Sengupta, Jayasree.

In: Contraception, Vol. 63, No. 6, 2001, p. 335-342.

Research output: Contribution to journalArticle

Ghosh, D, Dhawan, L, Lalitkumar, PGL, Wong, V, Rosario, JF, Hendrickx, AG, Lasley, BL, Overstreet, JW & Sengupta, J 2001, 'Effect of vaginally administered (Ala8,13,18)-magainin II amide on the morphology of implantation stage endometrium in the rhesus monkey (Macaca mulatta)', Contraception, vol. 63, no. 6, pp. 335-342. https://doi.org/10.1016/S0010-7824(01)00211-6
Ghosh, D. ; Dhawan, Latika ; Lalitkumar, P. G L ; Wong, Viviana ; Rosario, J. F. ; Hendrickx, Andrew G ; Lasley, B. L. ; Overstreet, J. W. ; Sengupta, Jayasree. / Effect of vaginally administered (Ala8,13,18)-magainin II amide on the morphology of implantation stage endometrium in the rhesus monkey (Macaca mulatta). In: Contraception. 2001 ; Vol. 63, No. 6. pp. 335-342.
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