Effect of vaginally administered (Ala8,13,18)-magainin II amide on the morphology of implantation stage endometrium in the rhesus monkey (Macaca mulatta)

D. Ghosh, Latika Dhawan, P. G L Lalitkumar, Viviana Wong, J. F. Rosario, Andrew G Hendrickx, B. L. Lasley, J. W. Overstreet, Jayasree Sengupta

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Intravaginal administration of an anti-microbial agent, (Ala8,13,18)-magainin II amide, during blastocyst implantation inhibits pregnancy establishment in a dose-related manner in the rhesus monkey (Macaca mulatta). In the present study, mated female rhesus monkeys were vaginally inserted with tampons containing vehicle (Group 1; n=5) and test agent (magainin, 0.5 mg/animal; Group 2; n=6) on cycle day 20. Endometrial tissue samples were collected on Cycle Day 24 from all monkeys and processed for morphometric and ultrastructural analysis. Concentrations of estradiol-17β, progesterone, and chorionic gonadotrophin in peripheral circulation were determined, which revealed that two monkeys in Group 1 were pregnant while no animals were pregnant in Group 2. Endometrial morphology, however, revealed histologic evidence of pregnancy in three out of the six magainin-treated animals. It appears that intra-vaginal administration of magainin II amide had a marginal effect on the implantation stage endometrium and the initiation of the implantation process in the rhesus monkey.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalContraception
Volume63
Issue number6
DOIs
StatePublished - 2001

Keywords

  • Endometrium
  • Implantation
  • Magainin
  • Morphology
  • Rhesus monkey

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

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    Ghosh, D., Dhawan, L., Lalitkumar, P. G. L., Wong, V., Rosario, J. F., Hendrickx, A. G., Lasley, B. L., Overstreet, J. W., & Sengupta, J. (2001). Effect of vaginally administered (Ala8,13,18)-magainin II amide on the morphology of implantation stage endometrium in the rhesus monkey (Macaca mulatta). Contraception, 63(6), 335-342. https://doi.org/10.1016/S0010-7824(01)00211-6