Abstract
To test the hypothesis that deferoxamine (DFO) may protect the lung against the acute toxicity of ozone (O3), male Sprague-Dawley rats (250 to 300 gm) were tracheally insufflated before exposure to O3 (2 ppm for 4 hours) with DFO (25 mg/kg), ferric-DFO, or sterile water. Measurements of bronchoalveolar lavage fluid (BALF) protein, ascorbate, hydrogen peroxide, and lipid hydroperoxides were made immediately after O3 exposure and 12 and 24 hours later. DFO (25 mg/kg) decreased BALF protein concentration (p < 0.05) and was associated with higher BALF ascorbate concentrations (p < 0.01) in O3-exposed animals. Ferrlc-DFO did not show these protective effects. No peroxides were found in BALF from any control or O3-exposed animals (detection limit: H2O2, 1 pmol; lipid hydroperoxides, 0.3 pmol). Higher DFO doses (50 mg/kg and 100 mg/kg) did not decrease BALF protein concentration immediately after O3 exposure or 12 hours later. Indeed, the highest dose was toxic to O3-exposed animals. Although DFO is able to protect against O3-induced lung damage in rats, apparently by chelating iron, its effective dose range appears to be narrow.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 502-509 |
| Number of pages | 8 |
| Journal | The Journal of Laboratory and Clinical Medicine |
| Volume | 121 |
| Issue number | 3 |
| State | Published - 1993 |
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ASJC Scopus subject areas
- Medicine(all)
- Pathology and Forensic Medicine
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Effect of tracheal insufflation of deferoxamine on acute ozone toxicity in rats. / Louie, Samuel; Arata, Michael A.; Offerdahl, Sharon D.; Halliwell, Barry.
In: The Journal of Laboratory and Clinical Medicine, Vol. 121, No. 3, 1993, p. 502-509.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of tracheal insufflation of deferoxamine on acute ozone toxicity in rats
AU - Louie, Samuel
AU - Arata, Michael A.
AU - Offerdahl, Sharon D.
AU - Halliwell, Barry
PY - 1993
Y1 - 1993
N2 - To test the hypothesis that deferoxamine (DFO) may protect the lung against the acute toxicity of ozone (O3), male Sprague-Dawley rats (250 to 300 gm) were tracheally insufflated before exposure to O3 (2 ppm for 4 hours) with DFO (25 mg/kg), ferric-DFO, or sterile water. Measurements of bronchoalveolar lavage fluid (BALF) protein, ascorbate, hydrogen peroxide, and lipid hydroperoxides were made immediately after O3 exposure and 12 and 24 hours later. DFO (25 mg/kg) decreased BALF protein concentration (p < 0.05) and was associated with higher BALF ascorbate concentrations (p < 0.01) in O3-exposed animals. Ferrlc-DFO did not show these protective effects. No peroxides were found in BALF from any control or O3-exposed animals (detection limit: H2O2, 1 pmol; lipid hydroperoxides, 0.3 pmol). Higher DFO doses (50 mg/kg and 100 mg/kg) did not decrease BALF protein concentration immediately after O3 exposure or 12 hours later. Indeed, the highest dose was toxic to O3-exposed animals. Although DFO is able to protect against O3-induced lung damage in rats, apparently by chelating iron, its effective dose range appears to be narrow.
AB - To test the hypothesis that deferoxamine (DFO) may protect the lung against the acute toxicity of ozone (O3), male Sprague-Dawley rats (250 to 300 gm) were tracheally insufflated before exposure to O3 (2 ppm for 4 hours) with DFO (25 mg/kg), ferric-DFO, or sterile water. Measurements of bronchoalveolar lavage fluid (BALF) protein, ascorbate, hydrogen peroxide, and lipid hydroperoxides were made immediately after O3 exposure and 12 and 24 hours later. DFO (25 mg/kg) decreased BALF protein concentration (p < 0.05) and was associated with higher BALF ascorbate concentrations (p < 0.01) in O3-exposed animals. Ferrlc-DFO did not show these protective effects. No peroxides were found in BALF from any control or O3-exposed animals (detection limit: H2O2, 1 pmol; lipid hydroperoxides, 0.3 pmol). Higher DFO doses (50 mg/kg and 100 mg/kg) did not decrease BALF protein concentration immediately after O3 exposure or 12 hours later. Indeed, the highest dose was toxic to O3-exposed animals. Although DFO is able to protect against O3-induced lung damage in rats, apparently by chelating iron, its effective dose range appears to be narrow.
UR - http://www.scopus.com/inward/record.url?scp=0027479155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027479155&partnerID=8YFLogxK
M3 - Article
C2 - 8445299
AN - SCOPUS:0027479155
VL - 121
SP - 502
EP - 509
JO - Translational Research
JF - Translational Research
SN - 1931-5244
IS - 3
ER -