Effect of tracheal insufflation of deferoxamine on acute ozone toxicity in rats

Samuel Louie, Michael A. Arata, Sharon D. Offerdahl, Barry Halliwell

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To test the hypothesis that deferoxamine (DFO) may protect the lung against the acute toxicity of ozone (O3), male Sprague-Dawley rats (250 to 300 gm) were tracheally insufflated before exposure to O3 (2 ppm for 4 hours) with DFO (25 mg/kg), ferric-DFO, or sterile water. Measurements of bronchoalveolar lavage fluid (BALF) protein, ascorbate, hydrogen peroxide, and lipid hydroperoxides were made immediately after O3 exposure and 12 and 24 hours later. DFO (25 mg/kg) decreased BALF protein concentration (p < 0.05) and was associated with higher BALF ascorbate concentrations (p < 0.01) in O3-exposed animals. Ferrlc-DFO did not show these protective effects. No peroxides were found in BALF from any control or O3-exposed animals (detection limit: H2O2, 1 pmol; lipid hydroperoxides, 0.3 pmol). Higher DFO doses (50 mg/kg and 100 mg/kg) did not decrease BALF protein concentration immediately after O3 exposure or 12 hours later. Indeed, the highest dose was toxic to O3-exposed animals. Although DFO is able to protect against O3-induced lung damage in rats, apparently by chelating iron, its effective dose range appears to be narrow.

Original languageEnglish (US)
Pages (from-to)502-509
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume121
Issue number3
StatePublished - 1993

Fingerprint

Insufflation
Deferoxamine
Ozone
Toxicity
Rats
Bronchoalveolar Lavage Fluid
Fluids
Animals
Lipid Peroxides
Lung
Proteins
Poisons
Peroxides
Chelation
Hydrogen Peroxide
Sprague Dawley Rats
Limit of Detection
Iron
Water

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Effect of tracheal insufflation of deferoxamine on acute ozone toxicity in rats. / Louie, Samuel; Arata, Michael A.; Offerdahl, Sharon D.; Halliwell, Barry.

In: The Journal of Laboratory and Clinical Medicine, Vol. 121, No. 3, 1993, p. 502-509.

Research output: Contribution to journalArticle

Louie, Samuel ; Arata, Michael A. ; Offerdahl, Sharon D. ; Halliwell, Barry. / Effect of tracheal insufflation of deferoxamine on acute ozone toxicity in rats. In: The Journal of Laboratory and Clinical Medicine. 1993 ; Vol. 121, No. 3. pp. 502-509.
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