Efeito tópico de iodo-povidona 0,5% comparado com natamicina 5% em ceratites fúngicas causadas por Fusarium solani em um modelo animal de coelhos: Estudo piloto

Translated title of the contribution: Effect of topical 0.5% povidone-iodine compared to 5% natamycin in fungal keratitis caused by Fusarium solani in a rabbit model: A pilot study

Lauro Augusto de Oliveira, Thales Takeo Takata, Alvio Isao Shiguematsu, Luiz Alberto Soares Melo, Olga Fischman Gompertz, Luciene Barbosa de Sousa, Mark J Mannis

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Purpose: To evaluate the efficacy of topical administration of 0.5% povidone-iodine in experimental Fusarium solani keratitis in rabbits. Methods: Fungal keratitis caused by Fusarium solani was induced in the right eye of 24 New Zealand rabbits. The rabbits were randomly divided into 3 different treatment groups: Group I (povidone-iodine) - treated with topical 0.5% povidone-iodine; Group II (natamycin) - treated with topical 5% natamycin; and Group III (control) - treated with topical saline solution. In all groups the rabbits were treated for three days and then sacrificed. The corneas were excised, macerated and immersed in 10 mL BHI. Culture samples were plated daily on Sabouraud's agar for 7 days, and the number of colony-forming units (CFU) was counted. The rabbits were clinically evaluated during the treatment period. Results: The povidone-iodine and natamycin groups demonstrated better efficacy than the control group based on the number of rabbits with no colonies growing. However, there were no statistically significant differences between the three groups when the number of CFU was analyzed (p>0.05). Conclusion: Our study demonstrates important methodological considerations in the use of in vivo animal models for the testing of antifungal agents. Using this sample size and methodology of counting CFU, topical 0.5% povidone-iodine demonstrated no benefit in the treatment of experimental Fusarium solani when compared with topical 5% natamycin.

Translated title of the contributionEffect of topical 0.5% povidone-iodine compared to 5% natamycin in fungal keratitis caused by Fusarium solani in a rabbit model: A pilot study
Original languageUndefined/Unknown
Pages (from-to)860-864
Number of pages5
JournalArquivos Brasileiros de Oftalmologia
Volume71
Issue number6
StatePublished - Nov 2008

Keywords

  • Antifungal agents/administration
  • Antifungal agents/therapeutic use
  • Dosage
  • Eye infections
  • Fungal/drug therapy
  • Fusarium
  • Keratitis/microbiology
  • Natamycin/administration
  • Natamycin/therapeutic use
  • Povidone-iodine/administration
  • Povidoneiodine/therapeutic use

ASJC Scopus subject areas

  • Ophthalmology

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