Effect of superfusate albumin on single capillary hydraulic conductivity

V. H. Huxley, F. E. Curry

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

It has been proposed that albumin interacts with the endothelial cell surface to form part of the molecular filter at the capillary wall. Previous investigations of the 'protein effect' have been limited to sites accessible to albumin from the capillary lumen. We tested for a specific interaction of albumin with the ablumenal surface of the capillary wall. The hydraulic conductivity (L(p)) of single capillaries in frog mesentery, perfused initially with albumin-free Ringer, was reduced when albumin (concentration > 1 mg/ml) was added to the superfusate (mean fractional reduction 0.56 ± 0.05 SE, n = 15). A similar reduction was measured when the mesothelial barrier to water and solute flows between the superfusate and the albuminal surface of the capillary was destroyed. When the albumin was extensively dialyzed against Ringer to remove small vasoactive molecules, no change in the fractional reduction of L(p) was observed. L(p) was reduced to a minimum value in any capillary when the albumin concentration on both sides of the capillary wall was >1 mg/ml. Our data conform to the hypothesis that albumin modifies structures throughout the capillary wall to maintain normal permeability. We predict that extravascular albumin reduces the ability of a Ringer perfusate to increase permeability in many isolated perfused organs.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume252
Issue number2
StatePublished - 1987
Externally publishedYes

Fingerprint

Albumins
Permeability
Mesentery
Anura
Endothelial Cells
Water
Proteins

ASJC Scopus subject areas

  • Physiology

Cite this

Effect of superfusate albumin on single capillary hydraulic conductivity. / Huxley, V. H.; Curry, F. E.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 252, No. 2, 1987.

Research output: Contribution to journalArticle

@article{7fb425a7a2fb455d99d2c65ccb3e88fa,
title = "Effect of superfusate albumin on single capillary hydraulic conductivity",
abstract = "It has been proposed that albumin interacts with the endothelial cell surface to form part of the molecular filter at the capillary wall. Previous investigations of the 'protein effect' have been limited to sites accessible to albumin from the capillary lumen. We tested for a specific interaction of albumin with the ablumenal surface of the capillary wall. The hydraulic conductivity (L(p)) of single capillaries in frog mesentery, perfused initially with albumin-free Ringer, was reduced when albumin (concentration > 1 mg/ml) was added to the superfusate (mean fractional reduction 0.56 ± 0.05 SE, n = 15). A similar reduction was measured when the mesothelial barrier to water and solute flows between the superfusate and the albuminal surface of the capillary was destroyed. When the albumin was extensively dialyzed against Ringer to remove small vasoactive molecules, no change in the fractional reduction of L(p) was observed. L(p) was reduced to a minimum value in any capillary when the albumin concentration on both sides of the capillary wall was >1 mg/ml. Our data conform to the hypothesis that albumin modifies structures throughout the capillary wall to maintain normal permeability. We predict that extravascular albumin reduces the ability of a Ringer perfusate to increase permeability in many isolated perfused organs.",
author = "Huxley, {V. H.} and Curry, {F. E.}",
year = "1987",
language = "English (US)",
volume = "252",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Effect of superfusate albumin on single capillary hydraulic conductivity

AU - Huxley, V. H.

AU - Curry, F. E.

PY - 1987

Y1 - 1987

N2 - It has been proposed that albumin interacts with the endothelial cell surface to form part of the molecular filter at the capillary wall. Previous investigations of the 'protein effect' have been limited to sites accessible to albumin from the capillary lumen. We tested for a specific interaction of albumin with the ablumenal surface of the capillary wall. The hydraulic conductivity (L(p)) of single capillaries in frog mesentery, perfused initially with albumin-free Ringer, was reduced when albumin (concentration > 1 mg/ml) was added to the superfusate (mean fractional reduction 0.56 ± 0.05 SE, n = 15). A similar reduction was measured when the mesothelial barrier to water and solute flows between the superfusate and the albuminal surface of the capillary was destroyed. When the albumin was extensively dialyzed against Ringer to remove small vasoactive molecules, no change in the fractional reduction of L(p) was observed. L(p) was reduced to a minimum value in any capillary when the albumin concentration on both sides of the capillary wall was >1 mg/ml. Our data conform to the hypothesis that albumin modifies structures throughout the capillary wall to maintain normal permeability. We predict that extravascular albumin reduces the ability of a Ringer perfusate to increase permeability in many isolated perfused organs.

AB - It has been proposed that albumin interacts with the endothelial cell surface to form part of the molecular filter at the capillary wall. Previous investigations of the 'protein effect' have been limited to sites accessible to albumin from the capillary lumen. We tested for a specific interaction of albumin with the ablumenal surface of the capillary wall. The hydraulic conductivity (L(p)) of single capillaries in frog mesentery, perfused initially with albumin-free Ringer, was reduced when albumin (concentration > 1 mg/ml) was added to the superfusate (mean fractional reduction 0.56 ± 0.05 SE, n = 15). A similar reduction was measured when the mesothelial barrier to water and solute flows between the superfusate and the albuminal surface of the capillary was destroyed. When the albumin was extensively dialyzed against Ringer to remove small vasoactive molecules, no change in the fractional reduction of L(p) was observed. L(p) was reduced to a minimum value in any capillary when the albumin concentration on both sides of the capillary wall was >1 mg/ml. Our data conform to the hypothesis that albumin modifies structures throughout the capillary wall to maintain normal permeability. We predict that extravascular albumin reduces the ability of a Ringer perfusate to increase permeability in many isolated perfused organs.

UR - http://www.scopus.com/inward/record.url?scp=0023090488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023090488&partnerID=8YFLogxK

M3 - Article

C2 - 3492926

AN - SCOPUS:0023090488

VL - 252

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 2

ER -