Effect of starvation on the charging levels of transfer ribonucleic acid and total acceptor capacity in rat liver

Surendra T. Shenoy, Quinton Rogers

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In a study of the mechanism of regulation of protein synthesis in the liver cell during starvation, the hepatic concentrations of eighteen amino acids and charging levels of tRNA of four essential amino acids, viz., phenylalanine, isoleucine, tryptophan and leucine, were measured in rats fed or starved for 48 h. The charging levels, measured by the periodate oxidation method, ranged from 79-91% and 77-101% in the fed and starved rats, respectively, but neither charging levels nor amino acid concentrations were lowered in starvation. Hepatic levels of essential amino acids in the starved rats were well above the known Km values of aminoacyl synthetases, indicating thereby that the liver maintained sufficiently high amino acid concentrations during starvation to maintain the capacity to charge tRNA near V. However, the total acceptor capacity as measured for tRNAPhe was reduced during starvation. Since the acceptor capacity per mg tRNA was not changed, the drop in the total acceptor capacity indicated a drop in the total amount of biologically active tRNAPhe. These results suggest that (i) the mechanism of regulation of protein synthesis in the liver cell during starvation is not mediated through a decrease in amino acid concentrations or in charging levels (periodate-protected capacity) of tRNA, and that (ii) the reduced protein synthesis observed during starvation is attributed, at least in part, to a reduced total acceptor capacity of the liver cell sap.

Original languageEnglish (US)
Pages (from-to)218-227
Number of pages10
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume476
Issue number3
DOIs
StatePublished - Jun 3 1977

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Starvation
Transfer RNA
Liver
RNA, Transfer, Phe
Amino Acids
Essential Amino Acids
tryptophan-leucine
Transfer RNA Aminoacylation
Proteins
Isoleucine
Ligases
Phenylalanine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of starvation on the charging levels of transfer ribonucleic acid and total acceptor capacity in rat liver. / Shenoy, Surendra T.; Rogers, Quinton.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 476, No. 3, 03.06.1977, p. 218-227.

Research output: Contribution to journalArticle

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