Abstract
The objective of this study was to compare and investigate differences in glucose and lactate concentrations in sodium fluoride/potassium oxalate (NaF/Ox) plasma and serum in healthy cats and cats with metabolic disease. Glucose and lactate concentrations were determined in routinely processed serum and NaF/Ox plasma obtained from healthy (n = 30), hyperthyroid (n = 27) and diabetic (n = 30) cats, and in samples from 6 healthy cats stored at 25°C or 4°C for 0, 1, 2, 4, or 8 hours. The packed cell volume (PCV) of blood collected in NaF/Ox was compared with that of blood collected in EDTA. Mean glucose concentration was significantly (P <.05) lower in NaF/Ox plasma than in serum in all groups of cats, by 0.7-2.5 mmol/L (11-45 mg/dL); the difference was greater in cats with hyperglycemia. Mean lactate concentration was significantly higher in serum than in NaF/Ox plasma in all groups of cats, by 0.4-1.2 mmol/L (3.6-10.8 mg/dL); the difference was greater in hyperthyroid and diabetic cats. In vitro, only serum stored on the clot for ≥ 1hour at 25°C had significantly lower glucose and higher lactate concentrations. The PCV of NaF/Ox-anticoagulated blood was lower than that of EDTA-anticoagulated blood, by 7.0% ± 1.4% (P <.01). In conclusion, collection of feline blood in NaF/Ox was necessary to prevent in vitro increases in lactate concentration; however, NaF/Ox artifactually decreased plasma glucose concentration because of RBC shrinkage. The PCV should not be determined on blood collected in NaF/Ox.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 22-28 |
| Number of pages | 7 |
| Journal | Veterinary Clinical Pathology |
| Volume | 29 |
| Issue number | 1 |
| State | Published - 2000 |
Keywords
- Feline
- Glucose
- Hyperglycemia
- Lactate
- Preanalytical error
- Sample collection
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- veterinary(all)