Effect of Specimen Collection and Storage on Blood Glucose and Lactate Concentrations in Healthy, Hyperthyroid and Diabetic Cats

Mary M Christopher, Sharron O'Neill

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The objective of this study was to compare and investigate differences in glucose and lactate concentrations in sodium fluoride/potassium oxalate (NaF/Ox) plasma and serum in healthy cats and cats with metabolic disease. Glucose and lactate concentrations were determined in routinely processed serum and NaF/Ox plasma obtained from healthy (n = 30), hyperthyroid (n = 27) and diabetic (n = 30) cats, and in samples from 6 healthy cats stored at 25°C or 4°C for 0, 1, 2, 4, or 8 hours. The packed cell volume (PCV) of blood collected in NaF/Ox was compared with that of blood collected in EDTA. Mean glucose concentration was significantly (P <.05) lower in NaF/Ox plasma than in serum in all groups of cats, by 0.7-2.5 mmol/L (11-45 mg/dL); the difference was greater in cats with hyperglycemia. Mean lactate concentration was significantly higher in serum than in NaF/Ox plasma in all groups of cats, by 0.4-1.2 mmol/L (3.6-10.8 mg/dL); the difference was greater in hyperthyroid and diabetic cats. In vitro, only serum stored on the clot for ≥ 1hour at 25°C had significantly lower glucose and higher lactate concentrations. The PCV of NaF/Ox-anticoagulated blood was lower than that of EDTA-anticoagulated blood, by 7.0% ± 1.4% (P <.01). In conclusion, collection of feline blood in NaF/Ox was necessary to prevent in vitro increases in lactate concentration; however, NaF/Ox artifactually decreased plasma glucose concentration because of RBC shrinkage. The PCV should not be determined on blood collected in NaF/Ox.

Original languageEnglish (US)
Pages (from-to)22-28
Number of pages7
JournalVeterinary Clinical Pathology
Volume29
Issue number1
StatePublished - 2000

Keywords

  • Feline
  • Glucose
  • Hyperglycemia
  • Lactate
  • Preanalytical error
  • Sample collection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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