Effect of Rapid Shallow Breathing on the Distribution of 18O-Labeled Ozone Reaction Product in the Respiratory Tract of the Rat

Mario F. Alfaro, Lei Putney, Brian K. Tarkington, Gary E. Hatch, Dallas M. Hyde, Edward S Schelegle

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We examined the effect of breathing pattern on ozone reaction product content within the respiratory tract. Thirty-four anesthetized, male Wistar rats were exposed to oxygen-18 (18O)-labeled ozone at 1.0 ppm for 2 h using a dual-chamber, negative-pressure ventilation system. Frequency was set at 80 (n = 9), 120 (n = 7), 160 (n = 8), or 200 (n = 10) breaths per minute (bpm), while tidal volume (Vt) was set to provide a constant minute ventilation of 72.8 ml/min/100 g body weight. Airways sampled were from the midlevel trachea and the mainstem bronchi and parenchyma of the cranial and caudal right lobes. 18O content in each airway sample was quantified and normalized to surface area. Across frequencies, there was significantly greater (p < .05) 18O content in the trachea and bronchi (conducting airway epithelium) compared to the parenchyma sampling sites. Tracheal 18O content decreased between 80 and 160 bpm, but then underwent an increase at 200 bpm. In comparison, 18O content gradually increased between 80 and 200 bpm at the right cranial and caudal bronchi sites. Right cranial parenchymal 18O content decreased at 200 bpm compared to 80, 120, and 160 bpm. Right caudal parenchymal 18O content was relatively constant over all breathing frequencies. We concluded that the development of rapid shallow breathing from 80 to 160 bpm results in a reduced deposition of 03 in the trachea, while only mildly affecting to ozone deposition in parenchyma supplied by short and long airway paths.

Original languageEnglish (US)
Pages (from-to)77-85
Number of pages9
JournalInhalation Toxicology
Volume16
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Ozone
Bronchi
Trachea
Reaction products
Respiratory System
Rats
Respiration
Ventilation
Tidal Volume
Wistar Rats
Epithelium
Body Weight
Oxygen
Pressure
Sampling

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Effect of Rapid Shallow Breathing on the Distribution of 18O-Labeled Ozone Reaction Product in the Respiratory Tract of the Rat. / Alfaro, Mario F.; Putney, Lei; Tarkington, Brian K.; Hatch, Gary E.; Hyde, Dallas M.; Schelegle, Edward S.

In: Inhalation Toxicology, Vol. 16, No. 2, 02.2004, p. 77-85.

Research output: Contribution to journalArticle

Alfaro, Mario F. ; Putney, Lei ; Tarkington, Brian K. ; Hatch, Gary E. ; Hyde, Dallas M. ; Schelegle, Edward S. / Effect of Rapid Shallow Breathing on the Distribution of 18O-Labeled Ozone Reaction Product in the Respiratory Tract of the Rat. In: Inhalation Toxicology. 2004 ; Vol. 16, No. 2. pp. 77-85.
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