Abstract
Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 234-235 |
| Number of pages | 2 |
| Journal | Chinese Journal of Clinical Rehabilitation |
| Volume | 8 |
| Issue number | 2 |
| State | Published - Jan 2004 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Rehabilitation
Cite this
Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells. / Zhao, Min; Xu, Jian Zhong; Zhou, Qiang; Wang, Xu Quan.
In: Chinese Journal of Clinical Rehabilitation, Vol. 8, No. 2, 01.2004, p. 234-235.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells
AU - Zhao, Min
AU - Xu, Jian Zhong
AU - Zhou, Qiang
AU - Wang, Xu Quan
PY - 2004/1
Y1 - 2004/1
N2 - Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.
AB - Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.
UR - http://www.scopus.com/inward/record.url?scp=2442566792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442566792&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:2442566792
VL - 8
SP - 234
EP - 235
JO - Chinese Journal of Clinical Rehabilitation
JF - Chinese Journal of Clinical Rehabilitation
SN - 1671-5926
IS - 2
ER -