Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells

Min Zhao; Jian Zhong Xu; Qiang Zhou; Xu Quan Wang

Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells

Min Zhao, Jian Zhong Xu, Qiang Zhou, Xu Quan Wang

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.

Original language	English (US)
Pages (from-to)	234-235
Number of pages	2
Journal	Chinese Journal of Clinical Rehabilitation
Volume	8
Issue number	2
State	Published - Jan 2004
Externally published	Yes

ASJC Scopus subject areas

Rehabilitation

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@article{581d0f9773404319889514e4d32aabd5,

title = "Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells",

abstract = "Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.",

author = "Min Zhao and Xu, {Jian Zhong} and Qiang Zhou and Wang, {Xu Quan}",

year = "2004",

month = jan,

language = "English (US)",

volume = "8",

pages = "234--235",

journal = "Chinese Journal of Clinical Rehabilitation",

issn = "1671-5926",

publisher = "Zhongguo Lin Chuang Kang Fu",

number = "2",

}

TY - JOUR

T1 - Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells

AU - Zhao, Min

AU - Xu, Jian Zhong

AU - Zhou, Qiang

AU - Wang, Xu Quan

PY - 2004/1

Y1 - 2004/1

N2 - Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.

AB - Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.

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