Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells

Min Zhao, Jian Zhong Xu, Qiang Zhou, Xu Quan Wang

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Abstract

Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.

Original languageEnglish (US)
Pages (from-to)234-235
Number of pages2
JournalChinese Journal of Clinical Rehabilitation
Volume8
Issue number2
StatePublished - Jan 2004
Externally publishedYes

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ASJC Scopus subject areas

  • Rehabilitation

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