Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.
|Original language||English (US)|
|Number of pages||2|
|Journal||Chinese Journal of Clinical Rehabilitation|
|State||Published - Jan 2004|
ASJC Scopus subject areas