Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells

Min Zhao, Jian Zhong Xu, Qiang Zhou, Xu Quan Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.

Original languageEnglish (US)
Pages (from-to)234-235
Number of pages2
JournalChinese Journal of Clinical Rehabilitation
Volume8
Issue number2
StatePublished - Jan 2004
Externally publishedYes

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Electromagnetic Fields
Mesenchymal Stromal Cells
Bone Marrow
Cell Proliferation
Apoptosis
S Phase
Flow Cytometry
Control Groups

ASJC Scopus subject areas

  • Rehabilitation

Cite this

Effect of pulsed electromagnetic fields on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells. / Zhao, Min; Xu, Jian Zhong; Zhou, Qiang; Wang, Xu Quan.

In: Chinese Journal of Clinical Rehabilitation, Vol. 8, No. 2, 01.2004, p. 234-235.

Research output: Contribution to journalArticle

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N2 - Aim: To observe the effect of pulsed electromagnetic fields (PEMFs) on the cellular proliferation and cycle of the human bone marrow mesenchymal stem cells(MSCs). Methods: After human bone marrow MSCs were stimulated by the PEMFs (12Hz pulse burst, 1. 1mT peak, 8 h/d). Methyl thiazol tetrazolium(MTT) assay and flow cytometry were applied to measure the cellular proliferation, cycle and apoptosis. Results: PEMFs treatment accelerated cellular proliferation obviously. After five days' stimulation, the percentage of phase S[ (20.15 ± 2.10)% ] was increased about 135.4% compared with that of the control group[ (8.56 ± 1.13)% ]. The percentage of cellular apoptosis was also decreased from (0.060 ± 0.005)% to (0.020 ± 0.003)%. Conclusion: One of the important biological effect of PEMFs on the human bone marrow MSCs is to accelerate cellular proliferation and cycle.

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