Effect of phytate reduction of sorghum, through genetic modification, on iron and zinc availability as assessed by an in vitro dialysability bioaccessibility assay, Caco-2 cell uptake assay, and suckling rat pup absorption model

Johanita Kruger, John R N Taylor, Xiaogu Du, Fabiana F. De Moura, Bo Lönnerdal, André Oelofse

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Improved iron and zinc availability from sorghum, a commonly consumed staple, will benefit many malnourished communities in rural Africa burdened with high prevalence of iron and zinc deficiency. This research compared the effect of genetic phytate reduction in sorghum on iron and zinc bioaccessibility and uptake measured by in vitro dialysability and Caco-2 cell uptake assays to that of iron and zinc absorption measured by a suckling rat pup model. The phytate reduction (80-86%) in these sorghums significantly increased zinc availability. The Caco-2 cell method, but not the dialysability assay, proved useful in estimating zinc absorption. The measured increase in iron availability differed between the methods, possibly due to the effect of varying mineral (Ca, Fe, Zn, P) contents of the sorghums. This effect was most prominent in the iron uptake results. More research is needed to determine the effect of naturally occurring variations in mineral contents of sorghum on the iron uptake by Caco-2 cells.

Original languageEnglish (US)
Pages (from-to)1019-1025
Number of pages7
JournalFood Chemistry
Volume141
Issue number2
DOIs
StatePublished - 2013

Fingerprint

Phytic Acid
Sorghum
Caco-2 Cells
phytic acid
genetic engineering
suckling
pups
Zinc
Rats
Assays
Iron
zinc
Availability
iron
rats
assays
cells
Minerals
rural communities
staples

Keywords

  • Caco-2
  • Dialysability
  • Genetic modification
  • GM
  • GMO
  • Iron
  • Phytate
  • Rat model
  • Sorghum
  • Zinc

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Effect of phytate reduction of sorghum, through genetic modification, on iron and zinc availability as assessed by an in vitro dialysability bioaccessibility assay, Caco-2 cell uptake assay, and suckling rat pup absorption model. / Kruger, Johanita; Taylor, John R N; Du, Xiaogu; De Moura, Fabiana F.; Lönnerdal, Bo; Oelofse, André.

In: Food Chemistry, Vol. 141, No. 2, 2013, p. 1019-1025.

Research output: Contribution to journalArticle

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