Effect of Perinatal secondhand tobacco smoke exposure on in vivo and intrinsic airway structure/function in non-human primates

Jesse P. Joad, Kayleen S. Kott, John M. Bric, Janice L. Peake, Kent E Pinkerton

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Infants exposed to second hand smoke (SHS) experience more problems with wheezing. This study was designed to determine if perinatal SHS exposure increases intrinsic and/or in vivo airway responsiveness to methacholine and whether potential structural/cellular alterations in the airway might explain the change in responsiveness. Pregnant rhesus monkeys were exposed to filtered air (FA) or SHS (1 mg/m3 total suspended particulates) for 6 h/day, 5 days/week starting at 50 days gestational age. The mother/infant pairs continued the SHS exposures postnatally. At 3 months of age each infant: 1) had in vivo lung function measurements in response to inhaled methacholine, or 2) the right accessory lobe filled with agarose, precision-cut to 600 μm slices, and bathed in increasing concentrations of methacholine. The lumenal area of the central airway was determined using videomicrometry followed by fixation and histology with morphometry. In vivo tests showed that perinatal SHS increases baseline respiratory rate and decreases responsiveness to methacholine. Perinatal SHS did not alter intrinsic airway responsiveness in the bronchi. However in respiratory bronchioles, SHS exposure increased airway responsiveness at lower methacholine concentrations but decreased it at higher concentrations. Perinatal SHS did not change eosinophil profiles, epithelial volume, smooth muscle volume, or mucin volume. However it did increase the number of alveolar attachments in bronchi and respiratory bronchioles. In general, as mucin increased, airway responsiveness decreased. We conclude that perinatal SHS exposure alters in vivo and intrinsic airway responsiveness, and alveolar attachments.

Original languageEnglish (US)
Pages (from-to)339-344
Number of pages6
JournalToxicology and Applied Pharmacology
Volume234
Issue number3
DOIs
StatePublished - Feb 1 2009

Fingerprint

Tobacco Smoke Pollution
Tobacco
Smoke
Primates
Methacholine Chloride
Bronchioles
Mucins
Bronchi
Histology
Respiratory Sounds
Accessories
Respiratory Rate
Macaca mulatta
Eosinophils
Sepharose
Gestational Age
Smooth Muscle
Muscle
Air
Mothers

Keywords

  • Airway responsiveness
  • Alveolar attachments
  • Eosinophils
  • Epithelium
  • Lung slice
  • Mucin
  • Smooth muscle
  • Videomicrometry

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Effect of Perinatal secondhand tobacco smoke exposure on in vivo and intrinsic airway structure/function in non-human primates. / Joad, Jesse P.; Kott, Kayleen S.; Bric, John M.; Peake, Janice L.; Pinkerton, Kent E.

In: Toxicology and Applied Pharmacology, Vol. 234, No. 3, 01.02.2009, p. 339-344.

Research output: Contribution to journalArticle

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