Effect of particle diameter and surface composition on the spontaneous fusion of monolayer-protected gold nanoparticles with lipid bilayers

Reid C. Van Lehn, Prabhani U. Atukorale, Randy Carney, Yu Sang Yang, Francesco Stellacci, Darrell J. Irvine, Alfredo Alexander-Katz

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free energy calculations, experiments on unilamellar and multilamellar vesicles, and cell studies all support this hypothesis. Furthermore, we show that fusion is only favorable for AuNPs with core diameters below a critical size that depends on the monolayer composition.

Original languageEnglish (US)
Pages (from-to)4060-4067
Number of pages8
JournalNano Letters
Volume13
Issue number9
DOIs
StatePublished - Sep 11 2013
Externally publishedYes

Fingerprint

Lipid bilayers
Surface structure
Gold
lipids
Monolayers
Fusion reactions
fusion
gold
Nanoparticles
Unilamellar Liposomes
nanoparticles
Free energy
penetration
free energy
membranes
Membranes
cells
Chemical analysis
Experiments

Keywords

  • cell penetration
  • gold nanoparticle
  • lipid bilayer
  • membrane insertion
  • snorkeling
  • surface monolayer

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Effect of particle diameter and surface composition on the spontaneous fusion of monolayer-protected gold nanoparticles with lipid bilayers. / Van Lehn, Reid C.; Atukorale, Prabhani U.; Carney, Randy; Yang, Yu Sang; Stellacci, Francesco; Irvine, Darrell J.; Alexander-Katz, Alfredo.

In: Nano Letters, Vol. 13, No. 9, 11.09.2013, p. 4060-4067.

Research output: Contribution to journalArticle

Van Lehn, Reid C. ; Atukorale, Prabhani U. ; Carney, Randy ; Yang, Yu Sang ; Stellacci, Francesco ; Irvine, Darrell J. ; Alexander-Katz, Alfredo. / Effect of particle diameter and surface composition on the spontaneous fusion of monolayer-protected gold nanoparticles with lipid bilayers. In: Nano Letters. 2013 ; Vol. 13, No. 9. pp. 4060-4067.
@article{2fe364d64cb74c518fd66b66f18ce8a2,
title = "Effect of particle diameter and surface composition on the spontaneous fusion of monolayer-protected gold nanoparticles with lipid bilayers",
abstract = "Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free energy calculations, experiments on unilamellar and multilamellar vesicles, and cell studies all support this hypothesis. Furthermore, we show that fusion is only favorable for AuNPs with core diameters below a critical size that depends on the monolayer composition.",
keywords = "cell penetration, gold nanoparticle, lipid bilayer, membrane insertion, snorkeling, surface monolayer",
author = "{Van Lehn}, {Reid C.} and Atukorale, {Prabhani U.} and Randy Carney and Yang, {Yu Sang} and Francesco Stellacci and Irvine, {Darrell J.} and Alfredo Alexander-Katz",
year = "2013",
month = "9",
day = "11",
doi = "10.1021/nl401365n",
language = "English (US)",
volume = "13",
pages = "4060--4067",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Effect of particle diameter and surface composition on the spontaneous fusion of monolayer-protected gold nanoparticles with lipid bilayers

AU - Van Lehn, Reid C.

AU - Atukorale, Prabhani U.

AU - Carney, Randy

AU - Yang, Yu Sang

AU - Stellacci, Francesco

AU - Irvine, Darrell J.

AU - Alexander-Katz, Alfredo

PY - 2013/9/11

Y1 - 2013/9/11

N2 - Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free energy calculations, experiments on unilamellar and multilamellar vesicles, and cell studies all support this hypothesis. Furthermore, we show that fusion is only favorable for AuNPs with core diameters below a critical size that depends on the monolayer composition.

AB - Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free energy calculations, experiments on unilamellar and multilamellar vesicles, and cell studies all support this hypothesis. Furthermore, we show that fusion is only favorable for AuNPs with core diameters below a critical size that depends on the monolayer composition.

KW - cell penetration

KW - gold nanoparticle

KW - lipid bilayer

KW - membrane insertion

KW - snorkeling

KW - surface monolayer

UR - http://www.scopus.com/inward/record.url?scp=84884253530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884253530&partnerID=8YFLogxK

U2 - 10.1021/nl401365n

DO - 10.1021/nl401365n

M3 - Article

C2 - 23915118

AN - SCOPUS:84884253530

VL - 13

SP - 4060

EP - 4067

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 9

ER -