Effect of paeoniflorin on the expressions of ras and raf-1 in the synoviocytes of rats with collagen-induced arthritis

Ling Ling Zhang, Yin Chen, Jing Yu Chen, Qingtong Wang, Hua Xun Wu, Wei Wei

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3 Scopus citations

Abstract

Objective: To investigate the effect of paeoniflorin (Pae) on the expressions of Ras and Raf-1 in the synoviocytes of the rats with collagen-induced arthritis (CIA). Methods: After CIA was induced by type II collagen, rats were treated with oral Pae (25, 50 and 100 mg · kg-1). Synoviocytes were isolated and cultured with Pae at various concentrations. Ras and Raf-1 were detected by Western blot. Results: CIA induced paw swelling, increased arthritis index (AI) and decreased body weight. Pae diminished the paw swelling and AI at 25, 50 and 100 mg·kg-1, and increased the weight of CIA rats at 50 and 100 mg·kg-1. The expressions of Ras and Raf-1 in synoviocytes were increased in CIA rats, which reached the maximum at 20-28 d and declined from 42 d after induction of CIA. Pae at 50, 100 mg·kg-1 suppressed the expressions of Ras and Raf-1 in the synoviocytes in vivo. In cultured synoviocytes, Pae at 2. 5, 12. 5 and 62. 5 mg·L-1 also inhibited the expressions in vitro. Conclusion: The signals, Ras and Raf-1, are involved in synovitis of CIA rats. Pae can modulate the abnormal Ras-MAPK signal pathway via affecting the expression of Ras and Raf-1, which may be one of the mechanisms underlying its inhibition of synovitis.

Original languageEnglish (US)
Pages (from-to)375-379
Number of pages5
JournalChinese Journal of New Drugs
Volume17
Issue number5
StatePublished - May 1 2008
Externally publishedYes

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Keywords

  • Arthritis
  • Paeoniflorin
  • Signal transduction
  • Synoviocytes

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

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