Effect of oxygenated sterol compounds on human bone marrow granulocytic progenitor cells

P. C. Hoffman, Carol M Richman, R. C. Hsu, J. Chung, A. M. Scanu, S. Yachnin

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Oxygenated sterol compounds are potent inhibitors of sterol and DNA synthesis in mammalian cells. We studied the effects of oxygenated sterols on human marrow granulocytic progenitor cells in vitro (CFU-C). 25-Hydroxycholesterol was found to be a potent inhibitor of sterol synthesis in marrow mononuclear cells, with 50% inhibition occurring at approximately 10:- 7 M. This compound, as well as 6-ketocholestanol, 7-ketocholesterol, and 20α-hydroxycholesterol, also demonstrated marked inhibition of CFU-C proliferation. The latter effect, which was not a result of direct cytotoxicity of the compounds, was reversible by cholesterol, but not by mevalonic acid. We conclude that inhibition of sterol synthesis by oxygenated sterol compounds may be insufficient to explain their suppression of CFU-C proliferation.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalBlood
Volume57
Issue number1
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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    Hoffman, P. C., Richman, C. M., Hsu, R. C., Chung, J., Scanu, A. M., & Yachnin, S. (1981). Effect of oxygenated sterol compounds on human bone marrow granulocytic progenitor cells. Blood, 57(1), 164-169.