Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia

Ana Rita Nunes, Vedangi Sample, Yang Kevin Xiang, Emília C. Monteiro, Estelle Gauda, Jin Zhang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

The cAMP-protein kinase A (PKA) signaling pathway is involved in regulating the release of transmitters from neurons and other cells. Multiple phosphodiesterase (PDE) isoforms regulate this pathway, however, the pattern of isoform expression and stimulus response across tissues has not been fully characterized. Using fluorescent resonance energy transfer (FRET)-based imaging in primary superior cervical ganglia (SCG) neurons and real-time qPCR, we explored the role of PDE3 and PDE4 isoforms and oxygen tension in the activation of PKA and changes in gene expression. These primary neurons were infected with an adenovirus containing A-Kinase activity reporter (AKAR3) and assayed for responses to PDE inhibitors: rolipram (ROL, 1 μM), milrinone (MIL, 10 μM) and IBMX (100 μM), and adenylyl cyclase activator forskolin (FSK, 50 μM). Different PDE activity patterns were observed in different cells: high PDE4 activity (n = 3), high PDE3 activity (n = 3) and presence of activity of other PDEs (n = 3). Addition of PKA inhibitor H89 (10 μM) completely reversed the response. We further studied the effect of oxygen in the PKA activity induced by PDE inhibition. Both normoxia (20%O2/5%CO2) and hypoxia (0%O2/5%CO2) induced a similar increase in the FRET emission ratio (14.5 ± 0.8 and 14.7 ± 0.8, respectively). PDE3a, PDE4b and PDE4d isoforms mRNAs were highly expressed in the whole SCG with no modulation by hypoxia. CONCLUSION: Using a FRET-based PKA activity sensor, we show that primary SCG neurons can be used as a model system to dissect the contribution of different PDE isoforms in regulating cAMP/PKA signaling. The differential patterns of PDE regulation potentially represent subpopulations of ganglion cells with different physiological functions.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
Pages287-294
Number of pages8
Volume758
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume758
ISSN (Print)00652598

Fingerprint

Type 4 Cyclic Nucleotide Phosphodiesterase
Superior Cervical Ganglion
Phosphoric Diester Hydrolases
Cyclic AMP-Dependent Protein Kinases
Protein Isoforms
Oxygen
Neurons
Energy Transfer
Energy transfer
Milrinone
Rolipram
RNA Isoforms
1-Methyl-3-isobutylxanthine
Frequency shift keying
Phosphodiesterase Inhibitors
Colforsin
Protein Kinase Inhibitors
Adenylyl Cyclases
Adenoviridae
Gene expression

Keywords

  • Cyclic AMP (cAMP)
  • Hypoxia
  • Phosphodiesterases (PDE)
  • Protein kinase A (PKA)
  • Superior cervical ganglia (SCG)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nunes, A. R., Sample, V., Xiang, Y. K., Monteiro, E. C., Gauda, E., & Zhang, J. (2012). Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia. In Advances in Experimental Medicine and Biology (Vol. 758, pp. 287-294). (Advances in Experimental Medicine and Biology; Vol. 758). https://doi.org/10.1007/978-94-007-4584-1-39

Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia. / Nunes, Ana Rita; Sample, Vedangi; Xiang, Yang Kevin; Monteiro, Emília C.; Gauda, Estelle; Zhang, Jin.

Advances in Experimental Medicine and Biology. Vol. 758 2012. p. 287-294 (Advances in Experimental Medicine and Biology; Vol. 758).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nunes, AR, Sample, V, Xiang, YK, Monteiro, EC, Gauda, E & Zhang, J 2012, Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia. in Advances in Experimental Medicine and Biology. vol. 758, Advances in Experimental Medicine and Biology, vol. 758, pp. 287-294. https://doi.org/10.1007/978-94-007-4584-1-39
Nunes AR, Sample V, Xiang YK, Monteiro EC, Gauda E, Zhang J. Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia. In Advances in Experimental Medicine and Biology. Vol. 758. 2012. p. 287-294. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-94-007-4584-1-39
Nunes, Ana Rita ; Sample, Vedangi ; Xiang, Yang Kevin ; Monteiro, Emília C. ; Gauda, Estelle ; Zhang, Jin. / Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia. Advances in Experimental Medicine and Biology. Vol. 758 2012. pp. 287-294 (Advances in Experimental Medicine and Biology).
@inproceedings{63da36d42989442d90c816b7633de4f2,
title = "Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia",
abstract = "The cAMP-protein kinase A (PKA) signaling pathway is involved in regulating the release of transmitters from neurons and other cells. Multiple phosphodiesterase (PDE) isoforms regulate this pathway, however, the pattern of isoform expression and stimulus response across tissues has not been fully characterized. Using fluorescent resonance energy transfer (FRET)-based imaging in primary superior cervical ganglia (SCG) neurons and real-time qPCR, we explored the role of PDE3 and PDE4 isoforms and oxygen tension in the activation of PKA and changes in gene expression. These primary neurons were infected with an adenovirus containing A-Kinase activity reporter (AKAR3) and assayed for responses to PDE inhibitors: rolipram (ROL, 1 μM), milrinone (MIL, 10 μM) and IBMX (100 μM), and adenylyl cyclase activator forskolin (FSK, 50 μM). Different PDE activity patterns were observed in different cells: high PDE4 activity (n = 3), high PDE3 activity (n = 3) and presence of activity of other PDEs (n = 3). Addition of PKA inhibitor H89 (10 μM) completely reversed the response. We further studied the effect of oxygen in the PKA activity induced by PDE inhibition. Both normoxia (20{\%}O2/5{\%}CO2) and hypoxia (0{\%}O2/5{\%}CO2) induced a similar increase in the FRET emission ratio (14.5 ± 0.8 and 14.7 ± 0.8, respectively). PDE3a, PDE4b and PDE4d isoforms mRNAs were highly expressed in the whole SCG with no modulation by hypoxia. CONCLUSION: Using a FRET-based PKA activity sensor, we show that primary SCG neurons can be used as a model system to dissect the contribution of different PDE isoforms in regulating cAMP/PKA signaling. The differential patterns of PDE regulation potentially represent subpopulations of ganglion cells with different physiological functions.",
keywords = "Cyclic AMP (cAMP), Hypoxia, Phosphodiesterases (PDE), Protein kinase A (PKA), Superior cervical ganglia (SCG)",
author = "Nunes, {Ana Rita} and Vedangi Sample and Xiang, {Yang Kevin} and Monteiro, {Em{\'i}lia C.} and Estelle Gauda and Jin Zhang",
year = "2012",
doi = "10.1007/978-94-007-4584-1-39",
language = "English (US)",
isbn = "9789400745834",
volume = "758",
series = "Advances in Experimental Medicine and Biology",
pages = "287--294",
booktitle = "Advances in Experimental Medicine and Biology",

}

TY - GEN

T1 - Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia

AU - Nunes, Ana Rita

AU - Sample, Vedangi

AU - Xiang, Yang Kevin

AU - Monteiro, Emília C.

AU - Gauda, Estelle

AU - Zhang, Jin

PY - 2012

Y1 - 2012

N2 - The cAMP-protein kinase A (PKA) signaling pathway is involved in regulating the release of transmitters from neurons and other cells. Multiple phosphodiesterase (PDE) isoforms regulate this pathway, however, the pattern of isoform expression and stimulus response across tissues has not been fully characterized. Using fluorescent resonance energy transfer (FRET)-based imaging in primary superior cervical ganglia (SCG) neurons and real-time qPCR, we explored the role of PDE3 and PDE4 isoforms and oxygen tension in the activation of PKA and changes in gene expression. These primary neurons were infected with an adenovirus containing A-Kinase activity reporter (AKAR3) and assayed for responses to PDE inhibitors: rolipram (ROL, 1 μM), milrinone (MIL, 10 μM) and IBMX (100 μM), and adenylyl cyclase activator forskolin (FSK, 50 μM). Different PDE activity patterns were observed in different cells: high PDE4 activity (n = 3), high PDE3 activity (n = 3) and presence of activity of other PDEs (n = 3). Addition of PKA inhibitor H89 (10 μM) completely reversed the response. We further studied the effect of oxygen in the PKA activity induced by PDE inhibition. Both normoxia (20%O2/5%CO2) and hypoxia (0%O2/5%CO2) induced a similar increase in the FRET emission ratio (14.5 ± 0.8 and 14.7 ± 0.8, respectively). PDE3a, PDE4b and PDE4d isoforms mRNAs were highly expressed in the whole SCG with no modulation by hypoxia. CONCLUSION: Using a FRET-based PKA activity sensor, we show that primary SCG neurons can be used as a model system to dissect the contribution of different PDE isoforms in regulating cAMP/PKA signaling. The differential patterns of PDE regulation potentially represent subpopulations of ganglion cells with different physiological functions.

AB - The cAMP-protein kinase A (PKA) signaling pathway is involved in regulating the release of transmitters from neurons and other cells. Multiple phosphodiesterase (PDE) isoforms regulate this pathway, however, the pattern of isoform expression and stimulus response across tissues has not been fully characterized. Using fluorescent resonance energy transfer (FRET)-based imaging in primary superior cervical ganglia (SCG) neurons and real-time qPCR, we explored the role of PDE3 and PDE4 isoforms and oxygen tension in the activation of PKA and changes in gene expression. These primary neurons were infected with an adenovirus containing A-Kinase activity reporter (AKAR3) and assayed for responses to PDE inhibitors: rolipram (ROL, 1 μM), milrinone (MIL, 10 μM) and IBMX (100 μM), and adenylyl cyclase activator forskolin (FSK, 50 μM). Different PDE activity patterns were observed in different cells: high PDE4 activity (n = 3), high PDE3 activity (n = 3) and presence of activity of other PDEs (n = 3). Addition of PKA inhibitor H89 (10 μM) completely reversed the response. We further studied the effect of oxygen in the PKA activity induced by PDE inhibition. Both normoxia (20%O2/5%CO2) and hypoxia (0%O2/5%CO2) induced a similar increase in the FRET emission ratio (14.5 ± 0.8 and 14.7 ± 0.8, respectively). PDE3a, PDE4b and PDE4d isoforms mRNAs were highly expressed in the whole SCG with no modulation by hypoxia. CONCLUSION: Using a FRET-based PKA activity sensor, we show that primary SCG neurons can be used as a model system to dissect the contribution of different PDE isoforms in regulating cAMP/PKA signaling. The differential patterns of PDE regulation potentially represent subpopulations of ganglion cells with different physiological functions.

KW - Cyclic AMP (cAMP)

KW - Hypoxia

KW - Phosphodiesterases (PDE)

KW - Protein kinase A (PKA)

KW - Superior cervical ganglia (SCG)

UR - http://www.scopus.com/inward/record.url?scp=84870434643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870434643&partnerID=8YFLogxK

U2 - 10.1007/978-94-007-4584-1-39

DO - 10.1007/978-94-007-4584-1-39

M3 - Conference contribution

C2 - 23080174

AN - SCOPUS:84870434643

SN - 9789400745834

VL - 758

T3 - Advances in Experimental Medicine and Biology

SP - 287

EP - 294

BT - Advances in Experimental Medicine and Biology

ER -