Effect of open and laparoscopic surgery on cellular immunity in a swine model

Ninh T. Nguyen, James D. Luketich, Steve Schatz, Quoc Tran, Hung S Ho, Phil R. Schauer

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Immune suppression associated with trauma has been demonstrated to be proportional to the magnitude of injury. Laparoscopic surgery has been shown to produce a diminished stress response compared to open surgery. Postoperative immune function, specifically cellular immunity, may be better preserved after laparoscopic surgery compared to open surgery. The aim of this study was to examine the effect of open versus laparoscopic surgery on cellular immunity in a swine model. Twenty domestic female pigs were randomly selected for laparoscopic cholecystectomy (LC) or open cholecystectomy (OC). Cellular immune functions were evaluated with delayed-type hypersensitivity (DTH) skin test and serial phytohemoagglutinin (PHA)-induced T-cell proliferation of the peripheral blood. There was a significant reduction in PHA-induced T-cell proliferation in both LC and OC groups on days 1 and 3 compared to preoperative values (p < 0.05). The reduction of mitogen-induced T-cell proliferation after LC was significantly less than after OC on day 1 (p = 0.03). The mean DTH reaction was 29.7 ± 3.7 mm 2 in the LC group compared to 13.9 ± 1.2 mm 2 in the OC group (p < 0.001). There was no difference in postoperative white blood count values between the two groups. Suppression of cellular immunity occurred after both LC and OC. The magnitude and duration of impaired cellular immunity after laparoscopic surgery was less than after open surgery as measured by T-cell proliferation and DTH response.

Original languageEnglish (US)
Pages (from-to)176-180
Number of pages5
JournalSurgical Laparoscopy and Endoscopy
Volume9
Issue number3
DOIs
StatePublished - Jun 1999

Keywords

  • Cellular immunity
  • Immune suppression
  • Laparoscopic cholecystectomy
  • Pig

ASJC Scopus subject areas

  • Surgery

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