Effect of oncotic pressure of diaspirin cross-linked hemoglobin (DCLHb(TM)) on brain injury after temporary focal cerebral ischemia in rats

Daniel J. Cole, John C. Drummond, Piyush M. Patel, Jeffrey C. Nary, Richard Lee Applegate

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Previous studies have shown that diaspirin cross-linked hemoglobin (DCLHb(TM), 10 g/dL) decreases cerebral ischemia and the resultant injury in a dose-dependent manner, requiring large volumes of DCLHb(TM) for maximum efficacy. We assessed the effect of a more concentrated (20 g/dL) and more hyperoncotic preparation of DCLHb(TM) on cerebral infarction volume. immediately after middle cerebral artery occlusion, rats were randomized to one of the following groups: Control, hematocrit not manipulated; 10/Hb, hematocrit decreased to 30% with 10% DCLHb(TM) (oncotic pressure 43 mm Hg); 7.5/Alb, hematocrit decreased to 30% with 7.5% albumin (oncotic pressure 43 mm Hg); 20/Hb, the same dose of DCLHb(TM) (20%, oncotic pressure 129 mm Hg) as the 10/Hb group (half the volume); or 15/Alb, the same dose of albumin (15%, oncotic pressure 130 mm Hg) as the 7.5/Alb group (half the volume). After 90 min of ischemia, 72 h of reperfusion was allowed. Infarction volume (mm3, mean ± SD) was less in the DCLHb(TM) groups (10/Hb = 79 ± 17; 20/Hb = 51 ± 14) than the oncotically matched albumin groups (7.5/Alb = 124 ± 21; 15/Alb = 85 ± 18) and the Control group (135 ± 17) (P < 0.05). These data indicate that in this model of cerebral ischemia, DCLHb(TM) decreases ischemic brain injury more effectively than albumin, and that a hyperoncotic preparation of DCLHb(TM) is preferable.

Original languageEnglish (US)
Pages (from-to)342-347
Number of pages6
JournalAnesthesia and Analgesia
Volume83
Issue number2
DOIs
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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