Effect of nifA gene product on expression of lacZ under nifH promoter in Escherichia coli.

Q. T. Kong, Q. L. Wu, L. B. Jia, Michael Syvanen, E. C. Lin, S. J. Shen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Gene expression of the nitrogen fixation system from Klebsiello pneumonice was studied in Escherichia coli by using compatible plasmids as vectors. One constructed plasmid carried the nifH promoter fused to the structural gene for beta-galactosidase, lac Z. Another plasmid carried the promoter of a tetracycline-resistance gene fused to nifA. We found that anaerobic synthesis of beta-galactosidase was greatly enhanced by the presence of an active nifA gene, indicating that its product is a positive control factor for transcription of nifH. In addition, anaerobic expression of lacZ was repressed by ammonium or serine in the presence of nifA. Thus the regulatory mechanism under study is of physiological relevance.

Original languageEnglish (US)
Pages (from-to)1061-1070
Number of pages10
JournalScientia Sinica. Series B, Chemical, biological, agricultural, medical & earth sciences / Chung-kuo k"o hsueh yuan, chu pan
Volume25
Issue number10
StatePublished - Oct 1982
Externally publishedYes

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plasmid
Plasmids
beta-Galactosidase
Escherichia coli
gene
Genes
Tetracycline Resistance
Nitrogen Fixation
nitrogen fixation
Ammonium Compounds
Serine
gene expression
Transcription Factors
ammonium
Gene Expression
effect
product

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of nifA gene product on expression of lacZ under nifH promoter in Escherichia coli. / Kong, Q. T.; Wu, Q. L.; Jia, L. B.; Syvanen, Michael; Lin, E. C.; Shen, S. J.

In: Scientia Sinica. Series B, Chemical, biological, agricultural, medical & earth sciences / Chung-kuo k"o hsueh yuan, chu pan, Vol. 25, No. 10, 10.1982, p. 1061-1070.

Research output: Contribution to journalArticle

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AU - Kong, Q. T.

AU - Wu, Q. L.

AU - Jia, L. B.

AU - Syvanen, Michael

AU - Lin, E. C.

AU - Shen, S. J.

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AB - Gene expression of the nitrogen fixation system from Klebsiello pneumonice was studied in Escherichia coli by using compatible plasmids as vectors. One constructed plasmid carried the nifH promoter fused to the structural gene for beta-galactosidase, lac Z. Another plasmid carried the promoter of a tetracycline-resistance gene fused to nifA. We found that anaerobic synthesis of beta-galactosidase was greatly enhanced by the presence of an active nifA gene, indicating that its product is a positive control factor for transcription of nifH. In addition, anaerobic expression of lacZ was repressed by ammonium or serine in the presence of nifA. Thus the regulatory mechanism under study is of physiological relevance.

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