Effect of nerve growth factor on endothelial cell biology: Proliferation and adherence molecule expression on human dermal microvascular endothelial cells

S. K. Raychaudhuri, Siba P Raychaudhuri, Helena Weltman, Eugene M. Farber

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

In addition to its effect on the central nervous system, nerve growth factor (NGF) appears to play a key role in the initiation and maintenance of inflammation in many organs. NGF degranulates mast cells, recruits inflammatory cellular infiltrates and activates T cells. Extravascular migration of leukocytes is initially controlled by the interaction of cell surface adhesion molecules of leukocytes and endothelial cells. A marked upregulation of NGF in keratinocytes is also observed in conditions characterized by angiogenesis such as psoriasis and wound healing. In this study we investigated the role of NGF in inflammation by studying its effects on endothelial cell proliferation and intracellular adhesion molecule expression by endothelial cells. The effect of NGF on human dermal microvascular endothelial cell (HDMEC) proliferation was measured using the hexosaminidase assay. ICAM-1 expression on HDMEC was measured by ELISA. The function of ICAM-1 was assessed by adherence of peripheral blood mononuclear cells (PBMC) to HDMEC using 51Cr-labeled PBMC. There was a significant increase in proliferation of HDMEC stimulated with NGF as compared to unstimulated HDMEC (P < 0.001). NGF-neutralizing antibody decreased the mitogenic effect of NGF significantly (P < 0.05). NGF also increased ICAM expression on HDMEC as compared to unstimulated HDMEC (P < 0.05). NGF-neutralizing antibody decreased ICAM expression on NGF-stimulated HDMEC (P < 0.05). The percentage of PBMC adherence was higher in NGF-stimulated HDMEC (P < 0.001). Anti-ICAM antibody decreased PBMC adherence. In the study reported here, the role of NGF in two important aspects of inflammation, i.e. angiogenesis and inflammatory cell recruitment at the site of inflammation, was investigated.

Original languageEnglish (US)
Pages (from-to)291-295
Number of pages5
JournalArchives of Dermatological Research
Volume293
Issue number6
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Nerve Growth Factor
Cell Biology
Endothelial Cells
Cell Proliferation
Skin
Blood Cells
Inflammation
Intercellular Adhesion Molecule-1
Neutralizing Antibodies
Leukocytes
Hexosaminidases
Cell Adhesion Molecules
Keratinocytes
Psoriasis
Mast Cells
Wound Healing
Anti-Idiotypic Antibodies
Up-Regulation
Central Nervous System
Enzyme-Linked Immunosorbent Assay

Keywords

  • Adhesion molecule
  • Endothelial cell
  • Inflammation
  • NGF

ASJC Scopus subject areas

  • Dermatology

Cite this

Effect of nerve growth factor on endothelial cell biology : Proliferation and adherence molecule expression on human dermal microvascular endothelial cells. / Raychaudhuri, S. K.; Raychaudhuri, Siba P; Weltman, Helena; Farber, Eugene M.

In: Archives of Dermatological Research, Vol. 293, No. 6, 2001, p. 291-295.

Research output: Contribution to journalArticle

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